{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:03:10Z","timestamp":1785402190228,"version":"3.56.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172559"],"award-info":[{"award-number":["82172559"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2026,3,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>To evaluate the clinical performance of Hybribio\u2019s 14-type HPV real-time PCR with 16\/18 genotyping (HBRT-H14) and its risk stratification utility among women with normal cytology (NILM). From 2017 to 2020, a multicenter cohort enrolled 8,401 women aged 30\u201364 years with NILM cytology. Baseline HPV testing used HBRT-H14. Women positive for HPV 16\/18 were referred for colposcopy; follow-up was annual for 3 years or until the detection of cervical intraepithelial neoplasia grade 2 or worse (CIN2+). Analyses included 6,679 women who completed follow-up. Overall HPV positivity was 11.4%, including 2.3% HPV 16\/18. Over 3 years, sensitivity and specificity of HPV positivity for CIN2+ were 92.3% (95% confidence interval [CI]: 84.2\u201396.4) and 89.6% (88.8\u201390.3). For HPV 16\/18 positivity, sensitivity and specificity were 41.0% (30.8\u201352.1) and 98.2% (97.8\u201398.5). Three-year cumulative CIN2+ risk was 20.9% (15.2\u201328.1) for HPV 16\/18-positive women, 6.6% (4.9\u20138.9) for other types, and 0.1% (0.04\u20130.2) for HPV-negative women. HBRT-H14 shows strong clinical performance for detecting CIN2+, and HPV 16\/18 genotyping provides effective risk stratification among women with NILM cytology. Findings support integration of HBRT-H14 into HPV-based screening pathways with HPV 16\/18 genotyping and cytology triage of other types.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>This multicenter prospective study evaluated the Hybribio 14 high-risk HPV real-time PCR assay (HBRT-H14) in 8,401 women with normal (NILM) cytology under guideline-based follow-up. The assay showed high clinical sensitivity and a very low risk among HPV-negative women, and HPV 16\/18 genotyping provided clear risk stratification. These findings deliver large-scale, practice-oriented evidence supporting integration of HBRT-H14 into HPV-based screening pathways that use HPV 16\/18 genotyping with cytology triage of other types.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.01289-25","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T14:17:24Z","timestamp":1771510644000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["HPV testing with 16\/18 genotyping for risk stratification among women with normal cytology: a multicenter prospective cohort study from China"],"prefix":"10.1128","volume":"64","author":[{"given":"Jiangong","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y0j0j86","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Life Sciences, Northwestern Polytechnical University","place":["Xi'an, China"]},{"name":"Department of 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