{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T14:23:15Z","timestamp":1786371795612,"version":"build-2736575974"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Mycoplasma pneumoniae<\/jats:named-content>is a major human respiratory pathogen causing both upper and lower respiratory disease in humans of all ages, and it can also result in other serious extrapulmonary sequelae. A multilocus sequence typing (MLST) scheme for<jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>was developed based on the sequences of eight housekeeping genes (<jats:italic>ppa<\/jats:italic>,<jats:italic>pgm<\/jats:italic>,<jats:italic>gyrB<\/jats:italic>,<jats:italic>gmk<\/jats:italic>,<jats:italic>glyA<\/jats:italic>,<jats:italic>atpA<\/jats:italic>,<jats:italic>arcC<\/jats:italic>, and<jats:italic>adk<\/jats:italic>) and applied to 55<jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>clinical isolates and the two type strains M129 and FH. A total of 12 sequence types (STs) resulted for 57<jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>isolates tested, with a discriminatory index of 0.21 STs per isolate. The MLST loci used in this scheme were shown to be stable in 10 strains following 10 sequential subculture passages. Phylogenetic analysis of concatenated sequences of the eight loci indicated two distinct genetic clusters that were directly linked to multilocus variable-number tandem repeat analysis (MLVA) type. Genetic MLST clustering was confirmed by genomic sequence analysis, indicating that the MLST scheme developed in this study is representative of the genome. Furthermore, this MLST scheme was shown to be more discriminatory than both MLVA and P1 typing for the<jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>isolates examined, providing a method for further and more detailed analysis of observed epidemic peaks of<jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>infection. This scheme is supported by a public Web-based database (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/pubmlst.org\/mpneumoniae\">http:\/\/pubmlst.org\/mpneumoniae<\/jats:ext-link>).<\/jats:p>","DOI":"10.1128\/jcm.01301-15","type":"journal-article","created":{"date-parts":[[2015,7,23]],"date-time":"2015-07-23T01:40:23Z","timestamp":1437615623000},"page":"3195-3203","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae"],"prefix":"10.1128","volume":"53","author":[{"given":"Rebecca J.","family":"Brown","sequence":"first","affiliation":[{"name":"Cardiff University, School of Medicine, Department of Child Health, University Hospital of Wales, Cardiff, United Kingdom"},{"name":"Bacteriology Reference Department, Public Health England, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T. G.","family":"Holden","sequence":"additional","affiliation":[{"name":"University of St. Andrews, School of Medicine, Medical &amp; Biological Sciences, North Haugh, St. Andrews, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. Brad","family":"Spiller","sequence":"additional","affiliation":[{"name":"Cardiff University, School of Medicine, Department of Child Health, University Hospital of Wales, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria J.","family":"Chalker","sequence":"additional","affiliation":[{"name":"Bacteriology Reference Department, Public Health England, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.697-728.2004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003983"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.17.05.20072-en"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1176-3"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2004.05.003"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.17.06.20081-en"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.17.06.20084-en"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119170"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.2807\/esm.03.10.00095-en"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365540050164155"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268899003313"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.965-970.2000"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.2.447-449.1996"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268810000622"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00495-06"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01935-08"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2011.06.008"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02567-12"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2015.05.005"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00231"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46281-0"},{"key":"e_1_3_3_25_2","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N","year":"1987","unstructured":"Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406\u2013425.","journal-title":"Mol Biol Evol"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4832-3211-9.50009-7"},{"key":"e_1_3_3_28_2","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195135848.001.0001","volume-title":"Molecular evolution and phylogenetics","author":"Nei M","year":"2000","unstructured":"Nei M, Kumar S. 2000. Molecular evolution and phylogenetics. Oxford University Press, New York, NY."},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.2307\/2408678"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.07.015"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03437-13"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01910-14"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.3.1190-1193.2005"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02597-14"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04308-14"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.4103\/1119-3077.153247"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1086\/508458"},{"key":"e_1_3_3_39_2","first-page":"w14041","article-title":"Survey of macrolide-resistant Mycoplasma pneumoniae in children with community-acquired pneumonia in Switzerland","volume":"144","author":"Meyer Sauteur PM","year":"2014","unstructured":"Meyer Sauteur PM, Bleisch B, Voit A, Maurer FP, Relly C, Berger C, Nadal D, Bloemberg GV. 2014. Survey of macrolide-resistant Mycoplasma pneumoniae in children with community-acquired pneumonia in Switzerland. Swiss Med Wkly 144:w14041.","journal-title":"Swiss Med Wkly"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0643-z"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1196"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01301-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01301-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T04:15:44Z","timestamp":1717992944000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01301-15"}},"subtitle":[],"editor":[{"given":"N. A.","family":"Ledeboer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,10]]},"references-count":40,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1128\/JCM.01301-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01301-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10]]},"assertion":[{"value":"2015-05-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}