{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T14:14:15Z","timestamp":1784038455515,"version":"3.55.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid, reliable, and easy-to-use diagnostic assays for detection of Zaire ebolavirus (ZEBOV) are urgently needed. The goal of this study was to examine the agreement among emergency use authorization (EUA) tests for the detection of ZEBOV nucleic acids, including the BioFire FilmArray BioThreat (BT) panel, the FilmArray BT-E panel, and the NP2 and VP40 quantitative real-time reverse transcriptase (qRT) PCR assays from the Centers for Disease Control and Prevention (CDC). Specimens used in this study included whole blood spiked with inactivated ZEBOV at known titers and whole-blood, plasma, and urine clinical specimens collected from persons diagnosed with Ebola virus disease (EVD). The agreement for FilmArray and qRT-PCR results using contrived whole-blood specimens was 100% (6\/6 specimens) for each ZEBOV dilution from 4 \u00d7 10\n            <jats:sup>7<\/jats:sup>\n            to 4 \u00d7 10\n            <jats:sup>2<\/jats:sup>\n            50% tissue culture infective dose (TCID\n            <jats:sub>50<\/jats:sub>\n            )\/ml, as well as the no-virus negative-control sample. The limit of detection for FilmArray and qRT-PCR assays with inactivated ZEBOV, based on duplicate positive results, was determined to be 4 \u00d7 10\n            <jats:sup>2<\/jats:sup>\n            TCID\n            <jats:sub>50<\/jats:sub>\n            \/ml. Rates of agreement between FilmArray and qRT-PCR results for clinical specimens from patients with EVD were 85% (23\/27 specimens) for whole-blood specimens, 90% (18\/20 specimens) for whole-blood specimens tested by FilmArray testing and matched plasma specimens tested by qRT-PCR testing, and 85% (11\/13 specimens) for urine specimens. Among 60 specimens, eight discordant results were noted, with ZEBOV nucleic acids being detected only by FilmArray testing in four specimens and only by qRT-PCR testing in the remaining four specimens. These findings demonstrate that the rapid and easy-to-use FilmArray panels are effective tests for evaluating patients with EVD.\n          <\/jats:p>","DOI":"10.1128\/jcm.01317-15","type":"journal-article","created":{"date-parts":[[2015,7,9]],"date-time":"2015-07-09T02:15:17Z","timestamp":1436408117000},"page":"2956-2960","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Comparison of FilmArray and Quantitative Real-Time Reverse Transcriptase PCR for Detection of Zaire Ebolavirus from Contrived and Clinical Specimens"],"prefix":"10.1128","volume":"53","author":[{"given":"Timothy R.","family":"Southern","sequence":"first","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"},{"name":"Nebraska Public Health Laboratory, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori D.","family":"Racsa","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar G.","family":"Albari\u00f1o","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Fey","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven H.","family":"Hinrichs","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin N.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"},{"name":"Nebraska Public Health Laboratory, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicki L.","family":"Herrera","sequence":"additional","affiliation":[{"name":"Nebraska Public Health Laboratory, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony R.","family":"Sambol","sequence":"additional","affiliation":[{"name":"Nebraska Public Health Laboratory, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles E.","family":"Hill","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily L.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colleen S.","family":"Kraft","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA"},{"name":"Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelley","family":"Campbell","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara K.","family":"Sealy","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Schuh","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Ritchie","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"G. Marshall","family":"Lyon","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneesh K.","family":"Mehta","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay B.","family":"Varkey","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce S.","family":"Ribner","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kent P.","family":"Brantly","sequence":"additional","affiliation":[{"name":"Samaritan's Purse, Boone, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Str\u00f6her","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Iwen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"},{"name":"Nebraska Public Health Laboratory, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eileen M.","family":"Burd","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA"},{"name":"Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60667-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1404505"},{"key":"e_1_3_3_4_2","unstructured":"Centers for Disease Control and Prevention. 2015. 2014 Ebola outbreak in West Africa. http:\/\/www.cdc.gov\/vhf\/ebola\/outbreaks\/2014-west-africa\/index.html. Accessed 20 June 2015."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.8.4330-4341.2004"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/v1030441"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1409838"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ334"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.7326\/M15-0530"},{"key":"e_1_3_3_10_2","volume-title":"FilmArray\u2122 BioThreat-E instructions for use","author":"BioFire Defense","year":"2014","unstructured":"BioFire Defense. 2014. FilmArray\u2122 BioThreat-E instructions for use. BioFire Defense, Salt Lake City, UT. http:\/\/www.biofiredefense.com\/media\/RFIT-PRT-0302-FA-BioThreat-E-Instructions-for-Use.pdf."},{"key":"e_1_3_3_11_2","volume-title":"Ebola virus NP real-time RT-PCR assay: instructions for use","author":"Centers for Disease Control and Prevention","year":"2014","unstructured":"Centers for Disease Control and Prevention. 2014. Ebola virus NP real-time RT-PCR assay: instructions for use. Centers for Disease Control and Prevention, Atlanta, GA. http:\/\/www.fda.gov\/downloads\/MedicalDevices\/Safety\/EmergencySituations\/UCM418810.pdf."},{"key":"e_1_3_3_12_2","volume-title":"Ebola virus VP40 real-time RT-PCR assay: instructions for use","author":"Centers for Disease Control and Prevention","year":"2014","unstructured":"Centers for Disease Control and Prevention. 2014. Ebola virus VP40 real-time RT-PCR assay: instructions for use. Centers for Disease Control and Prevention, Atlanta, GA. http:\/\/www.fda.gov\/downloads\/MedicalDevices\/Safety\/EmergencySituations\/UCM418815.pdf."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00527-15"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2014.09.001"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12764"},{"key":"e_1_3_3_16_2","unstructured":"Centers for Disease Control and Prevention. 30 January 2015. Guidance for collection transport and submission of specimens for Ebola virus testing. http:\/\/www.cdc.gov\/vhf\/ebola\/healthcare-us\/laboratories\/specimens.html. Accessed 5 April 2015."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01317-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01317-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:21:46Z","timestamp":1646482906000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01317-15"}},"subtitle":[],"editor":[{"given":"A. J.","family":"McAdam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":15,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1128\/JCM.01317-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01317-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"2015-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-06-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}