{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T16:13:27Z","timestamp":1793808807033,"version":"4.6.1"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Fundacion Bunge y Born","award":["Subsidios 2012 para investigacion en enfermedad de Chagas"],"award-info":[{"award-number":["Subsidios 2012 para investigacion en enfermedad de Chagas"]}]},{"DOI":"10.13039\/501100003074","name":"MINCyT | Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["FITS-Chagas"],"award-info":[{"award-number":["FITS-Chagas"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"MINCyT | Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["FITS-Chagas"],"award-info":[{"award-number":["FITS-Chagas"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"MINCyT | Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT2012-1650"],"award-info":[{"award-number":["PICT2012-1650"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"MINCyT | Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT2015-3715"],"award-info":[{"award-number":["PICT2015-3715"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Chagas disease is caused by the protozoan parasite\n            <jats:named-content content-type=\"genus-species\">Trypanosoma cruzi<\/jats:named-content>\n            . Assessment of parasitological cure upon treatment with available drugs relies on achieving consistent negative results in conventional parasitological and serological tests, which may take years to assess. Here, we evaluated the use of a recombinant\n            <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n            antigen termed trypomastigote small surface antigen (TSSA) as an early serological marker of drug efficacy in\n            <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n            -infected children. A cohort of 78 pediatric patients born to\n            <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n            -infected mothers was included in this study. Only 39 of the children were infected with\n            <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n            , and they were immediately treated with trypanocidal drugs. Serological responses against TSSA were evaluated in infected and noninfected populations during the follow-up period using an in-house enzyme-linked immunosorbent assay (ELISA) and compared to conventional serological methods. Anti-TSSA antibody titers decreased significantly faster than anti-whole parasite antibodies detected by conventional serology both in\n            <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n            -infected patients undergoing effective treatment and in those not infected. The differential kinetics allowed a significant reduction in the required follow-up periods to evaluate therapeutic responses or to rule out maternal-fetal transmission. Finally, we present the case of a congenitally infected patient with an atypical course in whom TSSA provided an early marker for\n            <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n            infection. In conclusion, we showed that TSSA was efficacious both for rapid assessment of treatment efficiency and for early negative diagnosis in infants at risk of congenital\n            <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n            infection. Based upon these findings we propose the inclusion of TSSA for refining the posttherapeutic cure criterion and other diagnostic needs in pediatric Chagas disease.\n          <\/jats:p>","DOI":"10.1128\/jcm.01317-17","type":"journal-article","created":{"date-parts":[[2017,10,5]],"date-time":"2017-10-05T00:40:22Z","timestamp":1507164022000},"page":"3444-3453","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["The Trypomastigote Small Surface Antigen from Trypanosoma cruzi Improves Treatment Evaluation and Diagnosis in Pediatric Chagas Disease"],"prefix":"10.1128","volume":"55","author":[{"given":"Virginia","family":"Balouz","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas-Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECh), Universidad Nacional de San Mart\u00edn (UNSAM) and Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano J.","family":"Melli","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas-Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECh), Universidad Nacional de San Mart\u00edn (UNSAM) and Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romina","family":"Volcovich","sequence":"additional","affiliation":[{"name":"Servicio de Parasitolog\u00eda-Chagas, Hospital de Ni\u00f1os Dr. Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Moscatelli","sequence":"additional","affiliation":[{"name":"Servicio de Parasitolog\u00eda-Chagas, Hospital de Ni\u00f1os Dr. Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samanta","family":"Moroni","sequence":"additional","affiliation":[{"name":"Servicio de Parasitolog\u00eda-Chagas, Hospital de Ni\u00f1os Dr. Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Servicio de Parasitolog\u00eda-Chagas, Hospital de Ni\u00f1os Dr. Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Griselda","family":"Ballering","sequence":"additional","affiliation":[{"name":"Servicio de Parasitolog\u00eda-Chagas, Hospital de Ni\u00f1os Dr. Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Bisio","sequence":"additional","affiliation":[{"name":"Servicio de Parasitolog\u00eda-Chagas, Hospital de Ni\u00f1os Dr. Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s E.","family":"Ciocchini","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas-Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECh), Universidad Nacional de San Mart\u00edn (UNSAM) and Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6466-4138","authenticated-orcid":false,"given":"Carlos A.","family":"Buscaglia","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas-Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECh), Universidad Nacional de San Mart\u00edn (UNSAM) and Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Altcheh","sequence":"additional","affiliation":[{"name":"Servicio de Parasitolog\u00eda-Chagas, Hospital de Ni\u00f1os Dr. Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.11.003"},{"key":"e_1_3_3_3_2","first-page":"33","article-title":"Chagas disease in Latin America: an epidemiological update based on 2010 estimates","volume":"90","author":"World Health Organization (WHO)","year":"2015","unstructured":"World Health Organization (WHO). 2015. Chagas disease in Latin America: an epidemiological update based on 2010 estimates. Wkly Epidemiol Rec90:33\u201343.","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1907.121576"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.3.551"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001250"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMct1014204"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg338"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/324355"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1053-2498(00)00168-6"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2017.05.010"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003312"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.59.526"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00106-07"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.02.028"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000316"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)04128-1"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(93)90501-G"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1993.49.625"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00274-12"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760160006"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2004.71.594"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1502504"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1086\/648072"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004657"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-14-302"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix277"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02248-16"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2014.899150"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.10.001"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37897424410.x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011433"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120074"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005856"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002906"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2016.01.021"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00684-14"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05289-11"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M114.045906"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.qco.0000247592.21295.a5"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7946-7949.2001"},{"key":"e_1_3_3_42_2","first-page":"37","article-title":"Anti-F2\/3 antibodies as cure marker in children with congenital Trypanosoma cruzi infection","volume":"63","author":"Altcheh J","year":"2003","unstructured":"Altcheh J Corral R Biancardi MA Freilij H . 2003. Anti-F2\/3 antibodies as cure marker in children with congenital Trypanosoma cruzi infection. Medicina (B Aires)63:37\u201340. (In Spanish.)","journal-title":"Medicina (B Aires)"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2015.1056158"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002892"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1507574"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1313122"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2684"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1177\/1087057114552623"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19339"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2010-1172"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002000"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(89)90050-9"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01317-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01317-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:50:38Z","timestamp":1646473838000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01317-17"}},"subtitle":[],"editor":[{"given":"Michael J.","family":"Loeffelholz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":51,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1128\/JCM.01317-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01317-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"2017-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}