{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T20:15:39Z","timestamp":1773432939277,"version":"3.50.1"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An elevated vancomycin MIC is associated with poor outcomes in\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            bacteremia (SAB) and is reported in patients with methicillin-susceptible\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            (MSSA) bacteremia in the absence of vancomycin treatment. Here, using DNA microarray and phenotype analysis, we investigated the genetic predictors and accessory gene regulator (\n            <jats:italic>agr<\/jats:italic>\n            ) function and their relationship with elevated vancomycin MIC using blood culture isolates from a multicenter binational cohort of patients with SAB. Specific clonal complexes were associated with elevated (clonal complex 8 [CC8] [\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001]) or low (CC22 [\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001], CC88 [\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001], and CC188 [\n            <jats:italic>P<\/jats:italic>\n            = 0.002]) vancomycin MIC.\n            <jats:italic>agr<\/jats:italic>\n            dysfunction (\n            <jats:italic>P<\/jats:italic>\n            = 0.014) or\n            <jats:italic>agr<\/jats:italic>\n            genotype II (\n            <jats:italic>P<\/jats:italic>\n            = 0.043) were also associated with an elevated vancomycin MIC. Specific resistance and virulence genes were also linked to an elevated vancomycin MIC, including\n            <jats:italic>blaZ<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            = 0.002),\n            <jats:italic>sea<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001),\n            <jats:italic>clfA<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001),\n            <jats:italic>splA<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            = 0.001), and the arginine catabolic mobile element (ACME) locus (\n            <jats:italic>P<\/jats:italic>\n            = 0.02). These data suggest that inherent organism characteristics may explain the link between elevated vancomycin MICs and poor outcomes in patients with SAB, regardless of the antibiotic treatment received. A consideration of clonal specificity should be included in future research when attempting to ascertain treatment effects or clinical outcomes.\n          <\/jats:p>","DOI":"10.1128\/jcm.01320-14","type":"journal-article","created":{"date-parts":[[2014,7,17]],"date-time":"2014-07-17T04:46:18Z","timestamp":1405572378000},"page":"3384-3393","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Genetic and Molecular Predictors of High Vancomycin MIC in Staphylococcus aureus Bacteremia Isolates"],"prefix":"10.1128","volume":"52","author":[{"given":"Natasha E.","family":"Holmes","sequence":"first","affiliation":[{"name":"Austin Centre for Infection Research, Department of Infectious Diseases, Austin Health, Heidelberg, Victoria, Australia"},{"name":"Department of Medicine, University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Turnidge","sequence":"additional","affiliation":[{"name":"SA Pathology, Women's and Children's Hospital, North Adelaide, South Australia, Australia"},{"name":"Department of Paediatrics, Pathology and Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy J.","family":"Munckhof","sequence":"additional","affiliation":[{"name":"Infection Management Services, Princess Alexandra Hospital, Wolloongabba, Queensland, Australia"},{"name":"Department of Medicine, University of Queensland, St Lucia, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Owen","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, PathWest Laboratory Medicine\u2013WA, Royal Perth Hospital, Perth, Western Australia, Australia"},{"name":"Australian Collaborating Centre for Enterococcus and Staphylococcus Species (ACCESS) Typing and Research, School of Biomedical Sciences, Curtin University, Perth, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony M.","family":"Korman","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Monash Health, Clayton, Victoria, Australia"},{"name":"Department of Medicine, Monash University, Clayton, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew V. N.","family":"O'Sullivan","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales, Australia"},{"name":"Department of Medicine, University of Sydney, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tara L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Royal Hobart Hospital, Hobart, Tasmania, Australia"},{"name":"Department of Medicine, University of Tasmania, Hobart, Tasmania, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally A.","family":"Roberts","sequence":"additional","affiliation":[{"name":"Auckland District Health Board, Auckland, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanchia J. C.","family":"Warren","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Royal Hobart Hospital, Hobart, Tasmania, Australia"},{"name":"Department of Medicine, University of Tasmania, Hobart, Tasmania, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoffrey W.","family":"Coombs","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, PathWest Laboratory Medicine\u2013WA, Royal Perth Hospital, Perth, Western Australia, Australia"},{"name":"Australian Collaborating Centre for Enterococcus and Staphylococcus Species (ACCESS) Typing and Research, School of Biomedical Sciences, Curtin University, Perth, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Leen","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, PathWest Laboratory Medicine\u2013WA, Royal Perth Hospital, Perth, Western Australia, Australia"},{"name":"Australian Collaborating Centre for Enterococcus and Staphylococcus Species (ACCESS) Typing and Research, School of Biomedical Sciences, Curtin University, Perth, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Gao","sequence":"additional","affiliation":[{"name":"Austin Centre for Infection Research, Department of Infectious Diseases, Austin Health, Heidelberg, Victoria, Australia"},{"name":"Department of Microbiology, Austin Health, Heidelberg, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul D. 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Organism factors associated with elevated vancomycin minimum inhibitory concentration (MIC) in Staphylococcus aureus bacteraemia. 15th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI), poster no. P 16\u2013312. 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C.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":42,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1128\/JCM.01320-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01320-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9]]},"assertion":[{"value":"2014-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}