{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:49:21Z","timestamp":1762004961760,"version":"3.41.2"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K09013"],"award-info":[{"award-number":["17K09013"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K15226"],"award-info":[{"award-number":["15K15226"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated the utility of Western blot (WB) bands of\n            <jats:named-content content-type=\"genus-species\">Bartonella hensela<\/jats:named-content>\n            e in detecting anti-\n            <jats:named-content content-type=\"genus-species\">B. henselae<\/jats:named-content>\n            immunoglobulin M (IgM) for serodiagnosis of cat scratch disease (CSD). IgM band patterns were examined using sera from 92 patients clinically suspected of having CSD and from 130 healthy individuals. Positive WB bands were observed in 49 (53.5%) of the 92 patient sera. Three bands at 8 to 10, 31 to 35, and 70 kDa were regarded as relevant for\n            <jats:named-content content-type=\"genus-species\">B. henselae<\/jats:named-content>\n            because all of the positive sera yielded at least one of the three bands, and none of the healthy control sera showed reactivity to any of them. In contrast, the positive rate of the patient sera by conventional indirect fluorescence antibody assay (IFA) for\n            <jats:named-content content-type=\"genus-species\">B. henselae<\/jats:named-content>\n            IgM was 28.3% (26\/92) among the patients. These finding suggest that the IgM-WB assay, although cumbersome to perform, can be used for confirmatory diagnosis of CSD with no false positivity in the control sera. Purification of proteins in the specific bands may contribute to the development of an IgM enzyme-linked immunosorbent assay (IgM-ELISA) with improved specificity and sensitivity.\n          <\/jats:p>","DOI":"10.1128\/jcm.01322-17","type":"journal-article","created":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T15:25:16Z","timestamp":1509549916000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Utility of Bartonella henselae IgM Western Blot Bands for Serodiagnosis of Cat Scratch Disease"],"prefix":"10.1128","volume":"56","author":[{"given":"Ken-ichiro","family":"Otsuyama","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehiro","family":"Tsuneoka","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroka","family":"Yoshidomi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Yamaguchi University Hospital, Ube, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mio","family":"Haraguchi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Yanagihara","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuko","family":"Tokuda","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junzo","family":"Nojima","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoshi","family":"Ichihara","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"doi-asserted-by":"publisher","key":"e_1_3_2_2_2","DOI":"10.1007\/BF01708232"},{"doi-asserted-by":"publisher","key":"e_1_3_2_3_2","DOI":"10.1007\/s10156-002-0194-6"},{"doi-asserted-by":"publisher","key":"e_1_3_2_4_2","DOI":"10.1128\/CMR.10.2.203"},{"doi-asserted-by":"publisher","key":"e_1_3_2_5_2","DOI":"10.2105\/AJPH.83.12.1707"},{"doi-asserted-by":"publisher","key":"e_1_3_2_6_2","DOI":"10.1007\/s10156-002-0202-X"},{"doi-asserted-by":"publisher","key":"e_1_3_2_7_2","DOI":"10.1016\/0140-6736(92)92032-B"},{"doi-asserted-by":"publisher","key":"e_1_3_2_8_2","DOI":"10.1128\/jcm.34.9.2270-2274.1996"},{"doi-asserted-by":"publisher","key":"e_1_3_2_9_2","DOI":"10.11150\/kansenshogakuzasshi1970.72.801"},{"doi-asserted-by":"publisher","key":"e_1_3_2_10_2","DOI":"10.1128\/jcm.35.8.1931-1937.1997"},{"key":"e_1_3_2_11_2","first-page":"1004","article-title":"Comparison of in-house and commercial slides for detection by immunofluorescence of immunoglobulin G and M against Bartonella henselae and Bartonella quintana","volume":"9","author":"Maurin M","year":"2002","unstructured":"Maurin M, Rolain JM, Raoult D. 2002. Comparison of in-house and commercial slides for detection by immunofluorescence of immunoglobulin G and M against Bartonella henselae and Bartonella quintana. Clin Diagn Lab Immunol 9:1004\u20131009.","journal-title":"Clin Diagn Lab Immunol"},{"doi-asserted-by":"publisher","key":"e_1_3_2_12_2","DOI":"10.1007\/s10096-008-0601-8"},{"doi-asserted-by":"publisher","key":"e_1_3_2_13_2","DOI":"10.1086\/324162"},{"doi-asserted-by":"publisher","key":"e_1_3_2_14_2","DOI":"10.1128\/JCM.03009-15"},{"doi-asserted-by":"publisher","key":"e_1_3_2_15_2","DOI":"10.1007\/s10156-006-0454-Y"},{"doi-asserted-by":"publisher","key":"e_1_3_2_16_2","DOI":"10.1128\/IAI.66.12.5915-5920.1998"},{"doi-asserted-by":"publisher","key":"e_1_3_2_17_2","DOI":"10.1093\/ajcp\/108.2.202"},{"doi-asserted-by":"publisher","key":"e_1_3_2_18_2","DOI":"10.1016\/j.diagmicrobio.2007.07.017"},{"doi-asserted-by":"publisher","key":"e_1_3_2_19_2","DOI":"10.1016\/j.diagmicrobio.2012.06.028"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01322-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01322-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:13:25Z","timestamp":1646475205000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01322-17"}},"subtitle":[],"editor":[{"given":"Brad","family":"Fenwick","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1128\/JCM.01322-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01322-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"type":"print","value":"0095-1137"},{"type":"electronic","value":"1098-660X"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"2017-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01322-17"}}