{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:49:54Z","timestamp":1790902194843,"version":"4.1.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Opisthorchis viverrini<\/jats:italic>\n            is an important food-borne trematode in Southeast Asia. The infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. The aim of this study was to improve the sensitivity of the PCR-based diagnosis of\n            <jats:italic>O. viverrini<\/jats:italic>\n            infection. A new fecal DNA extraction protocol for the detection of\n            <jats:italic>O. viverrini<\/jats:italic>\n            DNA using cetyltrimethyl-ammoniumbromide to remove PCR inhibitor was used and compared with the commercial stool kit method. The sensitivity of the new test was 79.3%, compared with the 44.8% of the previous method (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01). PCR-positive tests identified several cases judged parasite negative by the parasitological method (28.6%), indicating the new test's advantage in the diagnosis of individuals with light infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.01323-07","type":"journal-article","created":{"date-parts":[[2007,11,15]],"date-time":"2007-11-15T01:54:48Z","timestamp":1195091688000},"page":"366-368","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Improvement of PCR for Detection of\n            <i>Opisthorchis viverrini<\/i>\n            DNA in Human Stool Samples"],"prefix":"10.1128","volume":"46","author":[{"given":"Kunyarat","family":"Duenngai","sequence":"first","affiliation":[{"name":"Department of Parasitology"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paiboon","family":"Sithithaworn","sequence":"additional","affiliation":[{"name":"Department of Parasitology"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umesha Kanasinakatte","family":"Rudrappa","sequence":"additional","affiliation":[{"name":"Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karunasagar","family":"Iddya","sequence":"additional","affiliation":[{"name":"Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thewarach","family":"Laha","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christen Rune","family":"Stensvold","sequence":"additional","affiliation":[{"name":"DBL\u2014Center for Health Research and Development, DK-2920 Charlottenlund, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Strandgaard","sequence":"additional","affiliation":[{"name":"DBL\u2014Center for Health Research and Development, DK-2920 Charlottenlund, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Vang","family":"Johansen","sequence":"additional","affiliation":[{"name":"DBL\u2014Center for Health Research and Development, DK-2920 Charlottenlund, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.12.4463-4470.2000"},{"key":"e_1_3_2_3_2","first-page":"17","volume":"32","year":"2001","unstructured":"Ando, K., P. Sithithaworn, C. Nuchjungreed, S. Tesana, T. Srisawangwong, W. Limviroj, and Y. Chinzei. 2001. Nucleotide sequence of mitochondrial CO I and ribosomal ITS II genes of Opisthorchis viverrini in northeast Thailand. Southeast Asian J. Trop. Med. Public Health32(Suppl. 2):17-22.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_4_2","first-page":"121","volume":"61","year":"1994","unstructured":"Anonymous. 1994. Infection with liver flukes (Opisthorchis viverrini, Opisthorchis felineus and Clonorchis sinensis). IARC Monogr. Eval. Carcinog. Risks Hum.61:121-175.","journal-title":"IARC Monogr. Eval. Carcinog. Risks Hum."},{"key":"e_1_3_2_5_2","first-page":"1","volume":"849","year":"1995","unstructured":"Anonymous. 1995. Control of foodborne trematode infection. WHO Tech. Rep. Ser.849:1-157.","journal-title":"WHO Tech. Rep. 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