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Due to their close relationship, reliable species identification within the\n                      <jats:italic toggle=\"yes\">Cd<\/jats:italic>\n                      SC is also challenging using MALDI-TOF mass spectrometry (MS) for fast and reliable identification of bacteria. This is relevant for human and veterinary medicine. However, the Bruker MALDI-Biotyper (MBT), a commonly used MALDI-TOF MS system, does not reflect the current\n                      <jats:italic toggle=\"yes\">Cd<\/jats:italic>\n                      SC taxonomy, which includes\n                      <jats:italic toggle=\"yes\">C. belfantii<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">C. ramonii<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">C. rouxii<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">C. silvaticum<\/jats:italic>\n                      as additional\n                      <jats:italic toggle=\"yes\">Corynebacterium<\/jats:italic>\n                      species in the\n                      <jats:italic toggle=\"yes\">Cd<\/jats:italic>\n                      SC. Therefore, the objective of the present study is to expand the commercial Bruker database version K (MBT\n                      <jats:sub>K<\/jats:sub>\n                      ) in order to achieve reliable identification for all currently known\n                      <jats:italic toggle=\"yes\">Corynebacterium<\/jats:italic>\n                      species within the\n                      <jats:italic toggle=\"yes\">Cd<\/jats:italic>\n                      SC. Furthermore, the database expansions were verified with a systematic formal validation procedure in accordance with the German guideline for species identification by MALDI-TOF MS. For this, 328 spectra made from 321 strains including all seven valid species of the\n                      <jats:italic toggle=\"yes\">Cd<\/jats:italic>\n                      SC were identified using the MBT\n                      <jats:sub>K<\/jats:sub>\n                      database and a customer-supplemented modified MBT\n                      <jats:sub>K<\/jats:sub>\n                      database. This custom database version included 33 reference spectra of the\n                      <jats:italic toggle=\"yes\">Cd<\/jats:italic>\n                      SC covering all member species. The results of the formal validations for both the commercial and the extended database, combined partly with a single signal analysis, provide a substantiated basis for the identification of bacteria of the\n                      <jats:italic toggle=\"yes\">Cd<\/jats:italic>\n                      SC. A key element for a fast up-to-date identification has been the exchange of user-generated sets of main spectrum profiles (MSPs) on the open access MALDI User Platform (MALDI-UP) catalog.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Bacteria belonging to the\n                        <jats:italic toggle=\"yes\">Corynebacterium<\/jats:italic>\n                        (\n                        <jats:italic toggle=\"yes\">C<\/jats:italic>\n                        .)\n                        <jats:italic toggle=\"yes\">diphtheriae<\/jats:italic>\n                        complex are relevant pathogens for humans and animals with zoonotic potential. Identification of the novel species of this complex described since 2018, that is,\n                        <jats:italic toggle=\"yes\">C. belfantii<\/jats:italic>\n                        ,\n                        <jats:italic toggle=\"yes\">C. rouxii<\/jats:italic>\n                        ,\n                        <jats:italic toggle=\"yes\">C. ramonii<\/jats:italic>\n                        , and\n                        <jats:italic toggle=\"yes\">C. silvaticum<\/jats:italic>\n                        , is possible using the widely approved MALDI-TOF mass spectrometry. This is important for targeted therapies, preventive measures, and epidemiological studies. Precise species identification has been achieved by using a supplemented Bruker MALDI reference database. Comprehensive validation of database entries meets the requirements for accurate pathogen identification in accordance with recognized guidelines. This approach to community-driven database expansion and validation is certainly a model for other bacterial species of interest to react to current taxonomic revisions in a short time frame.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.01324-25","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T14:18:46Z","timestamp":1779459526000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Up-to-date MALDI-TOF MS-based identification of the\n                    <i>Corynebacterium diphtheriae<\/i>\n                    species complex for improved 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