{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T14:54:18Z","timestamp":1781880858924,"version":"3.54.5"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2020,6,24]]},"abstract":"<jats:p>\n            Neosporosis has become a concern since it is associated with abortion in cattle. Currently,\n            <jats:italic>in situ<\/jats:italic>\n            diagnosis is determined through anamnesis, evaluation of the history, and perception of the clinical signs of the herd. There is no practical and noninvasive test adapted to a large number of samples, which represents a gap for the use of new approaches that provide information about infections and the risks of herds. Here, we performed a search in the\n            <jats:named-content content-type=\"genus-species\">Neospora caninum<\/jats:named-content>\n            genome by linear B-cell epitopes using immunoinformatic tools aiming to develop a chimeric protein with high potential to bind specifically to antibodies from infected cattle samples.\n          <\/jats:p>","DOI":"10.1128\/jcm.01343-19","type":"journal-article","created":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T14:47:26Z","timestamp":1589294846000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Chimeric Protein Designed by Genome-Scale Immunoinformatics Enhances Serodiagnosis of Bovine Neosporosis"],"prefix":"10.1128","volume":"58","author":[{"given":"Higor Sette","family":"Pereira","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa, Vi\u00e7osa, Minas Gerais, 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