{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:33:40Z","timestamp":1787016820379,"version":"build-2736575974"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004740","name":"Fondazione del Monte di Bologna e Ravenna","doi-asserted-by":"publisher","award":["ID ROL FdM\/2400"],"award-info":[{"award-number":["ID ROL FdM\/2400"]}],"id":[{"id":"10.13039\/501100004740","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The use of interferon gamma (IFN-\u03b3) release assays (IGRAs) for the diagnosis of tuberculosis (TB) infection in children is still under debate because of concerns about the immature immune response in children. The aim of this study was to investigate quantitative values of the QuantiFERON-TB Gold In-Tube (QFT-IT) test, a commercially available IGRA, in a large cohort of children screened for TB infection. A retrospective analysis was conducted on samples from 517 children aged 0 to 14 years old at the Pediatric Unit of S. Orsola-Malpighi University Hospital of Bologna (Italy); quantitative responses to QFT-IT stimuli were analyzed according to diagnosis and age. Elevated IFN-\u03b3 values in the QFT-IT nil (background) tube were statistically associated with diagnosis of active TB. Quantitative IFN-\u03b3 response to\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    -specific antigens (TB Ag) was not significantly different in children with active TB compared to those with latent TB infection (LTBI), even though the median values were higher in the first group. When children were grouped by age, those less than 5 years old produced significantly higher levels of IFN-\u03b3 in response to TB Ag if they had active TB (median 10 IU\/ml) than those with LTBI (median 1.96 IU\/ml). IFN-\u03b3 response to mitogen increased with age. The overall rate of indeterminate results was low (3.9%), and no indeterminate QFT-IT values were observed in active or latent TB patients. In conclusion, quantitative QFT-IT values could provide further information to clinicians to manage TB in children, and these observations could be transferred to the new version of the test, QuantiFERON-TB Gold Plus, which to date lacks data from the pediatric population.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01360-17","type":"journal-article","created":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T11:20:31Z","timestamp":1510744831000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Quantitative Analysis of Gamma Interferon Release Assay Response in Children with Latent and Active Tuberculosis"],"prefix":"10.1128","volume":"56","author":[{"given":"Giulia","family":"Lombardi","sequence":"first","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine-Microbiology Unit, S. Orsola-Malpighi University Hospital, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Petrucci","sequence":"additional","affiliation":[{"name":"Paediatric Unit, S. Orsola-Malpighi University Hospital, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Corsini","sequence":"additional","affiliation":[{"name":"Unit of Pediatric Emergency, S. Orsola-Malpighi University Hospital, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Letizia","family":"Bacchi Reggiani","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine, S. Orsola-Malpighi University Hospital, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Visciotti","sequence":"additional","affiliation":[{"name":"Paediatric Unit, S. Orsola-Malpighi University Hospital, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Bernardi","sequence":"additional","affiliation":[{"name":"Unit of Pediatric Emergency, S. Orsola-Malpighi University Hospital, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Paola","family":"Landini","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine-Microbiology Unit, S. Orsola-Malpighi University Hospital, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Cazzato","sequence":"additional","affiliation":[{"name":"Paediatric Unit, S. Orsola-Malpighi University Hospital, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Dal Monte","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine-Microbiology Unit, S. 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