{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:54:02Z","timestamp":1790506442611,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            During the 2000s, a new clonal group with resistances to ampicillin, streptomycin, sulfonamides, and tetracycline (ASSuT) emerged in Italy among strains of\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Typhimurium and its monophasic variant,\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            subspecies\n            <jats:italic>enterica<\/jats:italic>\n            serovar 4,[5],12:i:\u2212. The PulseNet Europe database allowed us to identify ASSuT strains of both\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium and its monophasic variant, isolated in Denmark and the United Kingdom, with the same or very closely related pulsed-field gel electrophoresis (PFGE) patterns as the Italian strains, suggesting that the ASSuT clonal group is circulating in different European countries. With the aim of analyzing the molecular basis of antibiotic resistance, resistance genes were identified and their localization was investigated in 66 ASSuT strains and, as controls, in 11 strains with different resistance patterns and PFGE profiles, belonging both to\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium and to its monophasic variant, isolated from humans in Italy, Denmark, and the United Kingdom. All the ASSuT strains were positive for the following resistance genes:\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-1<\/jats:sub>\n            ,\n            <jats:italic>strA<\/jats:italic>\n            -\n            <jats:italic>strB<\/jats:italic>\n            ,\n            <jats:italic>sul<\/jats:italic>\n            2, and\n            <jats:italic>tet<\/jats:italic>\n            (B). A localization experiment demonstrated that the ASSuT resistance genes are chromosomally located. This study confirms that a multidrug-resistant clonal group, ASSuT, of\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium and its monophasic variant has emerged and is circulating in Italy, Denmark, and the United Kingdom. Moreover, the results of this work demonstrate that the multidrug resistance in this clonal group of\n            <jats:italic>Salmonella<\/jats:italic>\n            strains is conferred by a new genomic island.\n          <\/jats:p>","DOI":"10.1128\/jcm.01371-09","type":"journal-article","created":{"date-parts":[[2010,4,22]],"date-time":"2010-04-22T02:27:09Z","timestamp":1271903229000},"page":"2103-2109","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Evidence for a Second Genomic Island Conferring Multidrug Resistance in a Clonal Group of Strains of\n            <i>Salmonella<\/i>\n            <i>enterica<\/i>\n            Serovar Typhimurium and its Monophasic Variant Circulating in Italy, Denmark, and the United Kingdom"],"prefix":"10.1128","volume":"48","author":[{"given":"Claudia","family":"Lucarelli","sequence":"first","affiliation":[{"name":"Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanita\u0300, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Dionisi","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanita\u0300, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia","family":"Torpdahl","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Villa","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanita\u0300, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Graziani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanita\u0300, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Hopkins","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Gastrointestinal, Emerging and Zoonotic Infections, Centre for Infections, London NW9 5EQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Threlfall","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Gastrointestinal, Emerging and Zoonotic Infections, Centre for Infections, London NW9 5EQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Caprioli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanita\u0300, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"Luzzi","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanita\u0300, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00196-1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.4.1581-1586.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2009.0911"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.4.846"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268803001936"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2821-2828.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/314733"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2006.05.005"},{"key":"e_1_3_2_10_2","unstructured":"Institute performance standards for antimicrobial susceptibility testing; 26th informational supplement. 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