{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:33:40Z","timestamp":1792010020628,"version":"4.4.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Infectious diarrhea can be classified based on its clinical presentation as noninflammatory or inflammatory disease. In developing countries, among inflammatory diarrhea cases,\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            is the most common cause, followed by\n            <jats:named-content content-type=\"genus-species\">Campylobacter<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            . Because the time frame in which treatment choices must be made is short and conventional stool cultures lack good sensitivity, there is a need for a rapid, sensitive, and inexpensive detection technique. The purpose of our study was to develop a multiplex real-time PCR procedure to simultaneously identify\n            <jats:named-content content-type=\"genus-species\">Campylobacter<\/jats:named-content>\n            spp.,\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            spp., and\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            spp. Primers were designed to amplify the\n            <jats:italic>invA<\/jats:italic>\n            ,\n            <jats:italic>ipaH<\/jats:italic>\n            , and 16S rRNA genes simultaneously in a single reaction to detect\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Campylobacter<\/jats:named-content>\n            , respectively. Using this approach, we correctly identified 102 of 103 strains of the targeted enteropathogens and 34 of 34 other pathogens. The melting temperatures were 82.96 \u00b1 0.05\u00b0C for\n            <jats:italic>invA<\/jats:italic>\n            , 85.56 \u00b1 0.28\u00b0C for\n            <jats:italic>ipaH<\/jats:italic>\n            , and 89.21 \u00b1 0.24\u00b0C for 16S rRNA. The limit of accurate quantification for the assay in stool samples was 10\n            <jats:sup>4<\/jats:sup>\n            CFU g\n            <jats:sup>\u22121<\/jats:sup>\n            ; however, the limit of detection was 10\n            <jats:sup>3<\/jats:sup>\n            CFU g\n            <jats:sup>\u22121<\/jats:sup>\n            . This assay is a simple, rapid, inexpensive, and reliable system for the practical detection of these three enteropathogens in clinical specimens.\n          <\/jats:p>","DOI":"10.1128\/jcm.01397-13","type":"journal-article","created":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T03:53:26Z","timestamp":1371095606000},"page":"2822-2829","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Multiplex Real-Time PCR for Detection of Campylobacter, Salmonella, and Shigella"],"prefix":"10.1128","volume":"51","author":[{"given":"F.","family":"Barletta","sequence":"first","affiliation":[{"name":"Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical, Lima, Per\u00fa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. H.","family":"Mercado","sequence":"additional","affiliation":[{"name":"Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical, Lima, Per\u00fa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lluque","sequence":"additional","affiliation":[{"name":"Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical, Lima, Per\u00fa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Centre de Recerca en Salut Internacional de Barcelona, Hospital Clinic\/Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer, Universitat de Barcelona, Barcelona, Spain"},{"name":"CIBERESP, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. G.","family":"Cleary","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, University of Texas School of Public Health, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. J.","family":"Ochoa","sequence":"additional","affiliation":[{"name":"Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical, Lima, Per\u00fa"},{"name":"Department of Epidemiology, University of Texas School of Public Health, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.07.050054"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncpgasthep1264"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2012.01.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.02.072"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02341-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-50-8-667"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2008.09.006"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01219-07"},{"key":"e_1_3_2_10_2","first-page":"248","article-title":"Detection of Shigellae from stools of dysentery patients by culture and polymerase chain reaction techniques","volume":"16","author":"Islam MS","year":"1998","unstructured":"IslamMSHossainMSHasanMKRahmanMMFuchsGMahalanabisDBaquiAHAlbertMJ. 1998. 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