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It normally maintains a commensal lifestyle, but can cause dermatitis and otitis in predisposed hosts, particularly in atopic dogs.\n                      <jats:italic toggle=\"yes\">M. pachydermatis<\/jats:italic>\n                      is genetically diverse, with strains clustering into at least three phylogroups based on molecular typing, a pattern we now confirm through whole-genome sequencing (WGS). Accurate species and strain-level identification is essential for understanding its epidemiology, pathogenic potential, and response to treatment. In this study, we established Fourier transform infrared (FTIR) spectroscopy as a rapid, cost-effective method for distinguishing\n                      <jats:italic toggle=\"yes\">M. pachydermatis<\/jats:italic>\n                      from other\n                      <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                      species, including\n                      <jats:italic toggle=\"yes\">M. globosa<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">M. furfur<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">M. restricta<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">M. sympodialis<\/jats:italic>\n                      . Within\n                      <jats:italic toggle=\"yes\">M. pachydermatis<\/jats:italic>\n                      , FTIR spectroscopy resolved even closely related strains with high accuracy, producing clusters congruent with multilocus sequence typing (MLST)- and WGS-based phylogeny. The incorporation of an artificial neural network classifier further enhanced the discriminatory power, enabling robust and automated strain assignment. These findings demonstrate the potential of FTIR spectroscopy as a practical tool for large-scale epidemiological surveillance of\n                      <jats:italic toggle=\"yes\">M. pachydermatis<\/jats:italic>\n                      and for clinical and veterinary applications where strain-level identification could inform treatment and management of\n                      <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                      -associated diseases.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Malassezia pachydermatis<\/jats:italic>\n                        is a yeast that commonly inhabits the skin and ear canals of mammals but can cause dermatitis and otitis in predisposed hosts, especially dogs with allergies or immunosuppression. This species displays substantial genetic diversity, with strains falling into distinct phylogroups that may differ in their biology and clinical significance. Determining these differences has typically required advanced molecular or genomic methods, which can be costly and time-consuming. In this study, we demonstrate that Fourier transform infrared spectroscopy can rapidly and accurately distinguish\n                        <jats:italic toggle=\"yes\">M. pachydermatis<\/jats:italic>\n                        from other\n                        <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                        species and resolve genetic groups within the species in a way that reflects whole-genome relationships. This capability offers a practical tool for investigating the epidemiology and inter-\/intraspecies diversity of\n                        <jats:italic toggle=\"yes\">M. pachydermatis<\/jats:italic>\n                        and for guiding targeted management of\n                        <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                        -associated diseases in both veterinary and, potentially, human medicine.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.01422-25","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:12:26Z","timestamp":1784556746000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Fourier transform infrared spectroscopy enables rapid strain typing in\n                    <i>M. pachydermatis<\/i>"],"prefix":"10.1128","volume":"64","author":[{"given":"Simon","family":"Kurmann","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02crff812","id-type":"ROR","asserted-by":"publisher"}],"name":"Section of Immunology, Vetsuisse Faculty, University of Zurich","place":["Zurich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5716-0561","authenticated-orcid":false,"given":"Marco A.","family":"Coelho","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Genetics and Microbiology, Duke University School of Medicine","place":["Durham, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5515-7625","authenticated-orcid":true,"given":"M\u00e1rcia","family":"David-Palma","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Genetics and Microbiology, Duke University School of Medicine","place":["Durham, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3195-8732","authenticated-orcid":true,"given":"Leyna","family":"D\u00edaz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052g8jq94","id-type":"ROR","asserted-by":"publisher"}],"name":"Veterinary Mycology Group, Department of Animal Health and Anatomy, Veterinary School, Universitat Aut\u00f2noma de Barcelona","place":["Bellaterra, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8404-0688","authenticated-orcid":true,"given":"Gemma","family":"Castell\u00e1","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052g8jq94","id-type":"ROR","asserted-by":"publisher"}],"name":"Veterinary Mycology Group, Department of Animal Health and Anatomy, Veterinary School, Universitat Aut\u00f2noma de Barcelona","place":["Bellaterra, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2244-5778","authenticated-orcid":true,"given":"F. 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