{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:18:12Z","timestamp":1791407892291,"version":"4.3.3"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000614","name":"Federation of European Microbiological Societies","doi-asserted-by":"publisher","award":["FEMS-RG-2015-0049"],"award-info":[{"award-number":["FEMS-RG-2015-0049"]}],"id":[{"id":"10.13039\/501100000614","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Magnusiomyces capitatus<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Saprochaete clavata<\/jats:named-content>\n            are members of the clade of arthroconidial yeasts that represent emerging opportunistic pulmonary pathogens in immunocompromised patients. Given that standard ribosomal DNA (rDNA) identification often provides confusing results, in this study, we analyzed 34 isolates with the goal of finding new genetic markers for classification using multilocus sequencing and amplified fragment length polymorphism (AFLP). The interspecific similarity obtained using rDNA markers (the internal transcribed spacer [ITS] and large subunit regions) was in the range of 96 to 99%, whereas that obtained using protein-coding loci (\n            <jats:italic>Rbp2<\/jats:italic>\n            ,\n            <jats:italic>Act<\/jats:italic>\n            , and\n            <jats:italic>Tef1\u03b1<\/jats:italic>\n            ) was lower at 89.4 to 95.2%. Ultimately,\n            <jats:italic>Rbp2<\/jats:italic>\n            was selected as the best marker for species distinction. On the basis of cloned ITS data, some strains proved to be misidentified in comparison with the identities obtained with phenotypic characters, protein sequences, and AFLP profiles, indicating that different copies of the ribosomal operon were present in a single species. Antifungal susceptibility testing revealed that voriconazole had the lowest MIC against\n            <jats:named-content content-type=\"genus-species\">M. capitatus<\/jats:named-content>\n            , while amphotericin B had the lowest MIC against\n            <jats:italic>S. clavata<\/jats:italic>\n            . Both species exhibited\n            <jats:italic>in vitro<\/jats:italic>\n            resistance to fluconazole and micafungin.\n          <\/jats:p>","DOI":"10.1128\/jcm.01427-17","type":"journal-article","created":{"date-parts":[[2017,10,25]],"date-time":"2017-10-25T18:25:38Z","timestamp":1508955938000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Molecular Diagnostics of Arthroconidial Yeasts, Frequent Pulmonary Opportunists"],"prefix":"10.1128","volume":"56","author":[{"given":"Engin","family":"Kaplan","sequence":"first","affiliation":[{"name":"Division of Mycology, Department of Microbiology, Faculty of Medicine, University of \u00c7ukurova, Adana, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5206-2647","authenticated-orcid":false,"given":"Abdullah M. 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