{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:18:28Z","timestamp":1790072308470,"version":"4.0.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Mycoplasma pneumoniae<\/jats:named-content>\n            is a leading cause of community-acquired pneumonia (CAP) across patient populations of all ages. We have developed a loop-mediated isothermal amplification (LAMP) assay that enables rapid, low-cost detection of\n            <jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>\n            from nucleic acid extracts and directly from various respiratory specimen types. The assay implements calcein to facilitate simple visual readout of positive results in approximately 1 h, making it ideal for use in primary care facilities and resource-poor settings. The analytical sensitivity of the assay was determined to be 100 fg by testing serial dilutions of target DNA ranging from 1 ng to 1 fg per reaction, and no cross-reactivity was observed against 17 other\n            <jats:named-content content-type=\"genus-species\">Mycoplasma<\/jats:named-content>\n            species, 27 common respiratory agents, or human DNA. We demonstrated the utility of this assay by testing nucleic acid extracts (\n            <jats:italic>n<\/jats:italic>\n            = 252) and unextracted respiratory specimens (\n            <jats:italic>n<\/jats:italic>\n            = 72) collected during\n            <jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>\n            outbreaks and sporadic cases occurring in the United States from February 2010 to January 2014. The sensitivity of the LAMP assay was 88.5% tested on extracted nucleic acid and 82.1% evaluated on unextracted clinical specimens compared to a validated real-time PCR test. Further optimization and improvements to this method may lead to the availability of a rapid, cost-efficient laboratory test for\n            <jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>\n            detection that is more widely available to primary care facilities, ultimately facilitating prompt detection and appropriate responses to potential\n            <jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>\n            outbreaks and clusters within the community.\n          <\/jats:p>","DOI":"10.1128\/jcm.01431-15","type":"journal-article","created":{"date-parts":[[2015,7,16]],"date-time":"2015-07-16T02:30:18Z","timestamp":1437013818000},"page":"2970-2976","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Isothermal Detection of Mycoplasma pneumoniae Directly from Respiratory Clinical Specimens"],"prefix":"10.1128","volume":"53","author":[{"given":"Brianna L.","family":"Petrone","sequence":"first","affiliation":[{"name":"Pneumonia Response and Surveillance Laboratory, Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard J.","family":"Wolff","sequence":"additional","affiliation":[{"name":"Pneumonia Response and Surveillance Laboratory, Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra A.","family":"DeLaney","sequence":"additional","affiliation":[{"name":"Pneumonia Response and Surveillance Laboratory, Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen H.","family":"Diaz","sequence":"additional","affiliation":[{"name":"Pneumonia Response and Surveillance Laboratory, Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas M.","family":"Winchell","sequence":"additional","affiliation":[{"name":"Pneumonia Response and Surveillance Laboratory, Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/17.Supplement_1.S37"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2174\/15733963113099990009"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.697-728.2004"},{"key":"e_1_3_2_5_2","first-page":"1709","article-title":"Incidence of community-acquired pneumonia requiring hospitalization. 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