{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:52:53Z","timestamp":1789768373216,"version":"4.0.1"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diagnosis of\n                    <jats:named-content content-type=\"genus-species\">Propionibacterium acnes<\/jats:named-content>\n                    bone and joint infection is challenging due to the long cultivation time of up to 14 days. We retrospectively studied whether reducing the cultivation time to 7 days allows accurate diagnosis without losing sensitivity. We identified patients with at least one positive\n                    <jats:named-content content-type=\"genus-species\">P. acnes<\/jats:named-content>\n                    sample between 2005 and 2015 and grouped them into \u201cinfection\u201d and \u201cno infection.\u201d An infection was defined when at least two samples from the same case were positive. Clinical and microbiological data, including time to positivity for different cultivation methods, were recorded. We found 70 cases of proven\n                    <jats:named-content content-type=\"genus-species\">P. acnes<\/jats:named-content>\n                    infection with a significant faster median time to positivity of 6 days (range, 2 to 11 days) compared to 9 days in 47 cases with\n                    <jats:named-content content-type=\"genus-species\">P. acnes<\/jats:named-content>\n                    identified as a contamination (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.0001). In 15 of 70 (21.4%) patients with an infection, tissue samples were positive after day 7 and in 6 patients (8.6%) after day 10 when a blind subculture of the thioglycolate broth was performed. The highest sensitivity was detected for thioglycolate broth (66.3%) and the best positive predictive values for anaerobic agar plates (96.5%). A prolonged transportation time from the operating theater to the microbiological laboratory did not influence time to positivity of\n                    <jats:named-content content-type=\"genus-species\">P. acnes<\/jats:named-content>\n                    growth. By reducing the cultivation time to 7 days, false-negative diagnoses would increase by 21.4%; thus, we recommend that biopsy specimens from bone and joint infections be cultivated to detect\n                    <jats:named-content content-type=\"genus-species\">P. acnes<\/jats:named-content>\n                    for 10 days with a blind subculture at the end.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01435-16","type":"journal-article","created":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T22:29:35Z","timestamp":1476311375000},"page":"3043-3049","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":108,"title":["Optimal Length of Cultivation Time for Isolation of Propionibacterium acnes in Suspected Bone and Joint Infections Is More than 7 Days"],"prefix":"10.1128","volume":"54","author":[{"given":"Daniel A.","family":"Bossard","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Ledergerber","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick O.","family":"Zingg","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gerber","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annelies S.","family":"Zinkernagel","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Zbinden","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Achermann","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland"},{"name":"Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2009.01.023"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/588477"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.N.00881"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-012-0360-4"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2012.07.007"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31145"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00173-X"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05552-11"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.906461"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-004-0854-6"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02801.x"},{"key":"e_1_3_3_13_2","first-page":"477","article-title":"Severe infections caused by Propionibacterium acnes: an underestimated pathogen in late postoperative infections","volume":"69","author":"Jakab E","year":"1996","unstructured":"Jakab E, Zbinden R, Gubler J, Ruef C, von Graevenitz A, Krause M. 1996. 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