{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:05:48Z","timestamp":1790665548095,"version":"4.1.0"},"reference-count":11,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>An international multilaboratory collaborative study was conducted to develop standard media and consensus methods for the performance and quality control of antimicrobial susceptibility testing of<jats:named-content content-type=\"genus-species\">Mycoplasma pneumoniae<\/jats:named-content>,<jats:named-content content-type=\"genus-species\">Mycoplasma hominis<\/jats:named-content>, and<jats:named-content content-type=\"genus-species\">Ureaplasma urealyticum<\/jats:named-content>using broth microdilution and agar dilution techniques. A reference strain from the American Type Culture Collection was designated for each species, which was to be used for quality control purposes. Repeat testing of replicate samples of each reference strain by participating laboratories utilizing both methods and different lots of media enabled a 3- to 4-dilution MIC range to be established for drugs in several different classes, including tetracyclines, macrolides, ketolides, lincosamides, and fluoroquinolones. This represents the first multilaboratory collaboration to standardize susceptibility testing methods and to designate quality control parameters to ensure accurate and reliable assay results for mycoplasmas and ureaplasmas that infect humans.<\/jats:p>","DOI":"10.1128\/jcm.01439-12","type":"journal-article","created":{"date-parts":[[2012,8,23]],"date-time":"2012-08-23T02:42:29Z","timestamp":1345689749000},"page":"3542-3547","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["Standardized Methods and Quality Control Limits for Agar and Broth Microdilution Susceptibility Testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum"],"prefix":"10.1128","volume":"50","author":[{"given":"Ken B.","family":"Waites","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn B.","family":"Duffy","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile M.","family":"B\u00e9b\u00e9ar","sequence":"additional","affiliation":[{"name":"Unit\u00e9 sous Contrat Mycoplasmal and Chlamydial Infections in Humans, Universit\u00e9 Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Matlow","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah F.","family":"Talkington","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George E.","family":"Kenny","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Public Health, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Totten","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Public Health, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald J.","family":"Bade","sequence":"additional","affiliation":[{"name":"Microbial Research, Inc., Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Children's Memorial Hospital, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen K.","family":"Davidson","sequence":"additional","affiliation":[{"name":"FDA Center for Veterinary Medicine, Laurel, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia D.","family":"Shortridge","sequence":"additional","affiliation":[{"name":"bioM\u00e9rieux, Inc., Hazewood, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey L.","family":"Watts","sequence":"additional","affiliation":[{"name":"Pfizer Animal Health, Kalamazoo, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Brown","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Institute, Wilsonville, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"1","volume-title":"Antibiogram","author":"B\u00e9b\u00e9ar C","year":"2010","unstructured":"B\u00e9b\u00e9arC . 2010. 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