{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:23:47Z","timestamp":1791174227649,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated the capacity of the\n            <jats:italic>Mycoplasma agalactiae p40<\/jats:italic>\n            gene as a diagnostic marker for contagious agalactia in sheep by quantitative real-time PCR. The\n            <jats:italic>p40<\/jats:italic>\n            gene encodes an immunodominant adhesin that plays a key role in cytoadhesion of\n            <jats:italic>M. agalactiae<\/jats:italic>\n            . The assay was 100% specific, with an analytical sensitivity of 1 genome equivalent (GE), a quantification that is highly linear (\n            <jats:italic>R<\/jats:italic>\n            <jats:sup>2<\/jats:sup>\n            &gt; 0.992) and efficient (PCR efficiency, &gt;0.992) over a 6-log dynamic range, down to 10 GE. We evaluated the capacity of the assay to detect\n            <jats:italic>Mycoplasma agalactiae<\/jats:italic>\n            in 797 milk samples (373 raw sheep milk samples from refrigerated tanks of different farms and 424 milk samples from individual sheep of a flock positive for\n            <jats:italic>M. agalactiae<\/jats:italic>\n            ). In parallel, we also tested the samples by using microbiological isolation coupled with microscopy identification and by a PCR method recommended by the World Organization for Animal Health. While our assay was able to detect 57 (15.28%) positive samples of the 373 milk samples from different farms, identification by microbiological isolation coupled with microscopy detected only 36 (9.65%) samples, and the conventional PCR detected 31 (8.31%) samples. These findings showed that our assay based on the\n            <jats:italic>p40<\/jats:italic>\n            gene is more specific and sensitive for the detection of\n            <jats:italic>M. agalactiae<\/jats:italic>\n            in actual natural samples and, thus, can be a promising alternative tool for diagnosis and epidemiological studies of\n            <jats:italic>M. agalactiae<\/jats:italic>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/jcm.01442-08","type":"journal-article","created":{"date-parts":[[2008,11,20]],"date-time":"2008-11-20T01:44:02Z","timestamp":1227145442000},"page":"445-450","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["<i>Mycoplasma agalactiae p40<\/i>\n            Gene, a Novel Marker for Diagnosis of Contagious Agalactia in Sheep by Real-Time PCR: Assessment of Analytical Performance and In-House Validation Using Naturally Contaminated Milk Samples"],"prefix":"10.1128","volume":"47","author":[{"given":"Katari\u0301na","family":"Oravcova\u0301","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology and Microbiology"},{"name":"Food Research Institute, Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Lo\u0301pez-Enri\u0301quez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology and Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Rodri\u0301guez-La\u0301zaro","sequence":"additional","affiliation":[{"name":"Food Safety and Technology Group, Instituto Tecnolo\u0301gico Agrario de Castilla y Leo\u0301n (ITACyL), Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Herna\u0301ndez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology and Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.485-493.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2004.01.018"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.16.3.1062"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00058-I"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879200400126"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1995.tb07587.x"},{"key":"e_1_3_2_8_2","unstructured":"Resoluci\u00f3n de 24 de mayo de 2007 por la que desarrollan los programas sanitarios a realizar por las Agrupaciones de Defensa Sanitaria Ganaderas definidos en la Orden AYG\/1131\/2006 de 30 de junio. 2006"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5612-5621.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2004.11.007"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022979624333"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2003.02188.x"},{"key":"e_1_3_2_13_2","first-page":"145","volume":"104","year":"1998","unstructured":"Johansson, K. 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