{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:05:37Z","timestamp":1791227137247,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cystic fibrosis (CF) patients are predisposed to chronic respiratory infection by nonfermentative gram-negative bacilli, including\n            <jats:italic>Stenotrophomonas maltophilia. S. maltophilia<\/jats:italic>\n            is highly resistant to most antibiotics, with the exception of sulfamethoxazole-trimethoprim (SXT). SXT-resistant\n            <jats:italic>S. maltophilia<\/jats:italic>\n            has been reported, but the mechanism of resistance is not well defined. Repeated findings of suspected small-colony-variant (SCV)\n            <jats:italic>S. maltophilia<\/jats:italic>\n            isolates from the sputa of five CF patients were confirmed by partial 16S rRNA gene sequencing. The SCV\n            <jats:italic>S. maltophilia<\/jats:italic>\n            isolates were the only\n            <jats:italic>S. maltophilia<\/jats:italic>\n            isolates in these cultures, and none were clonally related. DNA fingerprint analysis confirmed that once established, the SCV\n            <jats:italic>S. maltophilia<\/jats:italic>\n            strains persisted. Nutritional studies of SCV\n            <jats:italic>S. maltophilia<\/jats:italic>\n            have suggested auxotrophy in hemin, methionine, and thymidine associated with resistance to multiple antibiotics, including SXT. The phenotypic switch from wild-type to SCV\n            <jats:italic>S. maltophilia<\/jats:italic>\n            was reproducible in vitro by exposure to SXT, suggesting that prolonged exposure to antibiotics may select for both the SCV\n            <jats:italic>S. maltophilia<\/jats:italic>\n            phenotype and SXT resistance by interference with the dihydrofolate reductase pathway. Recovery of SCV\n            <jats:italic>S. maltophilia<\/jats:italic>\n            from the sputum of CF patients has implications for both laboratory testing and patient management.\n          <\/jats:p>","DOI":"10.1128\/jcm.01444-06","type":"journal-article","created":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T01:53:38Z","timestamp":1164851618000},"page":"529-535","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Characterization of Small-Colony-Variant\n            <i>Stenotrophomonas maltophilia<\/i>\n            Isolated from the Sputum Specimens of Five Patients with Cystic Fibrosis"],"prefix":"10.1128","volume":"45","author":[{"given":"Scott W.","family":"Anderson","sequence":"first","affiliation":[{"name":"Therapeutic Development Network Core Laboratory for Cystic Fibrosis Microbiology"},{"name":"Microbiology Laboratory, Department of Laboratories and Pathology, Children's Hospital and Regional Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Stapp","sequence":"additional","affiliation":[{"name":"Therapeutic Development Network Core Laboratory for Cystic Fibrosis Microbiology"},{"name":"Microbiology Laboratory, Department of Laboratories and Pathology, Children's Hospital and Regional Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane L.","family":"Burns","sequence":"additional","affiliation":[{"name":"Therapeutic Development Network Core Laboratory for Cystic Fibrosis Microbiology"},{"name":"Division of Infectious Diseases, Immunology and Rheumatology, Department of Pediatrics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Qin","sequence":"additional","affiliation":[{"name":"Therapeutic Development Network Core Laboratory for Cystic Fibrosis Microbiology"},{"name":"Microbiology Laboratory, Department of Laboratories and Pathology, Children's Hospital and Regional Medical Center"},{"name":"Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.1999.0708"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.37-40.1995"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.10216"},{"key":"e_1_3_2_5_2","unstructured":"Clinical and Laboratory Standards Institute. 2006. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; M7-A7. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_2_6_2","unstructured":"Clinical and Laboratory Standards Institute. 2006. Performance standards for antimicrobial susceptibility testing; M100-S16. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.7.2180-2187.1992"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.1.57"},{"key":"e_1_3_2_9_2","first-page":"461","year":"2004","unstructured":"DiMango, E., and A. Prince. 2004. Pseudomonas aeruginosa and cystic fibrosis, p. 461-475. In A. A. Gershon, P. J. Hotez, and S. L. Katz (ed.), Krugman's infectious diseases of children, 11th ed. Mosby, Philadelphia, PA.","journal-title":"Krugman's infectious diseases of children"},{"key":"e_1_3_2_10_2","first-page":"914","volume":"166","year":"2002","unstructured":"Goss, C. H., K. Otto, M. L. Aitken, and G. D. Rubenfeld. 2002. Detecting Stenotrophomonas maltophilia does not reduce survival of patients with cystic fibrosis. Am. J. Crit. Care. Med.166:914-915.","journal-title":"Am. J. Crit. Care. 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Vindel, L. Maiz, F. Baquero, H. Escobar, and R. Canton. 2001. Persistence and variability of Stenotrophomonas maltophilia in cystic fibrosis patients, Madrid 1991-1998. Emerg. Infect. Dis.1:113-122.","journal-title":"Emerg. Infect. 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