{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:58:47Z","timestamp":1777514327942,"version":"3.51.4"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Proper identification of\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            by conventional methods remains problematic. The discriminatory power of the 16S rRNA gene, which can be considered the \u201cgold standard\u201d for molecular identification, is too low to differentiate\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            from closely related species such as\n            <jats:named-content content-type=\"genus-species\">Streptococcus pseudopneumoniae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Streptococcus mitis<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Streptococcus oralis<\/jats:named-content>\n            in the routine clinical laboratory. A 313-bp part of\n            <jats:italic>recA<\/jats:italic>\n            was selected on the basis of variability within the\n            <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n            group, showing &lt;95.8% interspecies homology. In addition, 6 signature nucleotides specific for\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            were identified within the 313-bp\n            <jats:italic>recA<\/jats:italic>\n            fragment. We show that\n            <jats:italic>recA<\/jats:italic>\n            analysis is a useful tool for proper identification to species level within the\n            <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n            group, in particular, for pneumococci.\n          <\/jats:p>","DOI":"10.1128\/jcm.01450-10","type":"journal-article","created":{"date-parts":[[2010,12,9]],"date-time":"2010-12-09T03:34:27Z","timestamp":1291865667000},"page":"523-527","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["<i>recA<\/i>\n            -Based PCR Assay for Accurate Differentiation of Streptococcus pneumoniae from Other Viridans Streptococci"],"prefix":"10.1128","volume":"49","author":[{"given":"A.","family":"Zbinden","sequence":"first","affiliation":[{"name":"University of Zurich, Institute of Medical Microbiology, Gloriastrasse 30\/32, CH-8006 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"K\u00f6hler","sequence":"additional","affiliation":[{"name":"University of Zurich, Institute of Medical Microbiology, Gloriastrasse 30\/32, CH-8006 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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