{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T04:28:28Z","timestamp":1780374508004,"version":"3.54.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The aminoglycosides streptomycin, amikacin, and kanamycin and the cyclic polypeptide capreomycin are all widely used in second-line therapy for patients who develop multidrug-resistant tuberculosis. We have characterized a set of 106 clinical isolates of\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            using phenotypic drug susceptibility testing (DST) to determine the extent of resistance to each agent and cross-resistance between agents. These results were compared with polymorphisms in the DNA sequences of ribosome-associated genes previously implicated in resistance and with the clinical outcomes of subjects from whom these isolates were obtained. Thirty-six (34%) of these isolates displayed resistance to one or more of these agents, and the majority of these (20 of 36) showed cross-resistance to one or more agents. Most (33 of 36) of the resistant isolates showed polymorphisms in the 16S ribosome components RpsL and\n            <jats:italic>rrs<\/jats:italic>\n            . Three resistant strains (3 of 36) were identified that had no known polymorphisms in ribosomal constituents. For kanamycin and streptomycin, molecular DST significantly outperformed phenotypic DST using the absolute concentration method for predicting 4-month sputum conversion (likelihood ratios of 4.0 and 2.0, respectively) and was equivalent to phenotypic DST using the National Committee for Clinical Laboratory Standards (NCCLS)-approved agar proportion method for estimating MIC (likelihood ratio, 4.0). These results offer insight into mechanisms of resistance and cross-resistance among these agents and suggest that the development of rapid molecular tests to distinguish polymorphisms would significantly enhance clinical utility of this important class of second-line antituberculosis drugs.\n          <\/jats:p>","DOI":"10.1128\/jcm.01476-09","type":"journal-article","created":{"date-parts":[[2009,12,24]],"date-time":"2009-12-24T03:20:22Z","timestamp":1261624822000},"page":"402-411","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Polymorphisms Associated with Resistance and Cross-Resistance to Aminoglycosides and Capreomycin in\n            <i>Mycobacterium tuberculosis<\/i>\n            Isolates from South Korean Patients with Drug-Resistant Tuberculosis"],"prefix":"10.1128","volume":"48","author":[{"given":"Laura E.","family":"Via","sequence":"first","affiliation":[{"name":"Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Nae","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soohee","family":"Hwang","sequence":"additional","affiliation":[{"name":"National Masan Tuberculosis Hospital, Masan, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeeun","family":"Bang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, Yonsei University, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Kyu","family":"Park","sequence":"additional","affiliation":[{"name":"National Masan Tuberculosis Hospital, Masan, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung Seok","family":"Kang","sequence":"additional","affiliation":[{"name":"National Masan Tuberculosis Hospital, Masan, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doosoo","family":"Jeon","sequence":"additional","affiliation":[{"name":"National Masan Tuberculosis Hospital, Masan, Republic of Korea"},{"name":"International Tuberculosis Research Center, Masan, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seon Yeong","family":"Min","sequence":"additional","affiliation":[{"name":"International Tuberculosis Research Center, Masan, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taegwon","family":"Oh","sequence":"additional","affiliation":[{"name":"International Tuberculosis Research Center, Masan, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, Yonsei University, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vignesh","family":"Rajan","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon Y.","family":"Wong","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isdore Chola","family":"Shamputa","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Carroll","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Goldfeder","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song A.","family":"Lee","sequence":"additional","affiliation":[{"name":"International Tuberculosis Research Center, Masan, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M.","family":"Holland","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seokyong","family":"Eum","sequence":"additional","affiliation":[{"name":"International Tuberculosis Research Center, Masan, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, Yonsei University, Wonju, Republic of 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