{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T17:09:57Z","timestamp":1788628197995,"version":"build-2803163510"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Nonsporulating molds (NSMs), especially basidiomycetes, have predominantly been reported as human pathogens responsible for allergic and invasive disease. Their conventional identification is problematic, as many isolates remain sterile in culture. Thus, inconclusive culture reports might adversely affect treatment decisions. The clinical significance of NSMs in pulmonary mycoses is poorly understood. We sequenced the internal transcribed spacer (ITS) region and D1\/D2 domain of the larger subunit (LSU) of 52 NSMs isolated from respiratory specimens. The basidiomycetes were the predominant NSMs, of which\n            <jats:named-content content-type=\"genus-species\">Schizophyllum commune<\/jats:named-content>\n            was the most common agent in allergic bronchopulmonary mycosis (ABPM), followed by\n            <jats:named-content content-type=\"genus-species\">Ceriporia lacerata<\/jats:named-content>\n            in invasive fungal disease.\n            <jats:named-content content-type=\"genus-species\">Porostereum spadiceum<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Phanaerochaete stereoides<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Neosartorya fischeri<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Marasmiellus palmivorus<\/jats:named-content>\n            were the other molds observed. Application of ITS and LSU region sequencing identified 92% of the isolates. The antifungal susceptibility data revealed that all basidiomycetes tested were susceptible to amphotericin B and resistant to caspofungin, fluconazole, and flucytosine. Except for 3 isolates of\n            <jats:named-content content-type=\"genus-species\">S. commune<\/jats:named-content>\n            and a solitary isolate of\n            <jats:named-content content-type=\"genus-species\">M. palmivorus<\/jats:named-content>\n            , all basidiomycetes had low MICs for itraconazole, posaconazole, and voriconazole. Basidiomycetes were isolated from patients with ABPM, invasive pulmonary mycosis\/pneumonia, or fungal balls. In addition, the majority of the basidiomycetes were isolated from patients with chronic respiratory disorders who were sensitized to one of the basidiomycetous fungi and demonstrated precipitating antibodies against the incriminating fungi, indicating an indolent tissue reaction. Thus, isolation of basidiomycetes from the lower respiratory tract could be significant, and it is important to monitor these patients in order to prevent subsequent lung damage.\n          <\/jats:p>","DOI":"10.1128\/jcm.01486-13","type":"journal-article","created":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T04:41:58Z","timestamp":1375332118000},"page":"3331-3337","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Clinical Significance and Molecular Characterization of Nonsporulating Molds Isolated from the Respiratory Tracts of Bronchopulmonary Mycosis Patients with Special Reference to Basidiomycetes"],"prefix":"10.1128","volume":"51","author":[{"given":"Pradeep Kumar","family":"Singh","sequence":"first","affiliation":[{"name":"Departments of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shallu","family":"Kathuria","sequence":"additional","affiliation":[{"name":"Departments of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kshitij","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Pulmonary Medicine, Vallabhbhai Patel Chest Institute, University of Delhi, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailendra Nath","family":"Gaur","sequence":"additional","affiliation":[{"name":"Pulmonary Medicine, Vallabhbhai Patel Chest Institute, University of Delhi, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques F.","family":"Meis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"},{"name":"Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuradha","family":"Chowdhary","sequence":"additional","affiliation":[{"name":"Departments of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0809.020073"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.925-928.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2675-2678.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01222-10"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01213-10"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03266.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00393569"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.8.1979-1983.1995"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01684-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(96)91436-X"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219780001431"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.7.1628-1632.1996"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2012.02190.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/18.3.305"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01863-12"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02943-12"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46016-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2008.11.012"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.40.2.217.219"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.2009-2011.2005"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2012.682320"},{"key":"e_1_3_2_23_2","first-page":"315","volume-title":"PCR protocols: a guide to methods and applications","author":"White TJ","year":"1990","unstructured":"WhiteTJBrunsTLeeSTaylorJ. 1990. 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