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Culture-based methods, thus far considered the \u201cgold standard\u201d for identifying bacterial microorganisms, require 24 to 48 h to provide a diagnosis. In addition, antimicrobial therapy is often started prior to clinical sample collection, thereby decreasing the probability of confirming the bacterial pathogen by culture-based methods. To enable a fast and accurate detection of the most important bacterial pathogens causing meningitis, namely,\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Haemophilus influenzae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Streptococcus agalactiae<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            , we evaluated a commercially available multiplex LightMix real-time PCR (RT-PCR) in 220 cerebrospinal fluid (CSF) specimens. The majority of CSF samples were collected by lumbar puncture, but we also included some CSF samples from patients with symptoms of meningitis from the neurology department that were recovered from shunts. CSF samples were analyzed by multiplex RT-PCR enabling a first diagnosis within a few hours after sample arrival at our institute. In contrast, bacterial identification took between 24 and 48 h by culture. Overall, a high agreement of bacterial identification between culture and multiplex RT-PCR was observed (99%). Moreover, multiplex RT-PCR enabled the detection of pathogens,\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 2),\n            <jats:named-content content-type=\"genus-species\">S. agalactiae<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 1), and\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 1), in four culture-negative samples. As a complement to classical bacteriological CSF culture, the LightMix RT-PCR assay proved to be valuable by improving the rapidity and accuracy of the diagnosis of bacterial meningitis.\n          <\/jats:p>","DOI":"10.1128\/jcm.01492-17","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T16:40:24Z","timestamp":1513183224000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Pathogen Identification by Multiplex LightMix Real-Time PCR Assay in Patients with Meningitis and Culture-Negative Cerebrospinal Fluid Specimens"],"prefix":"10.1128","volume":"56","author":[{"given":"Karoline","family":"Wagner","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burkard","family":"Springer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria P.","family":"Pires","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Keller","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040845"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(20)30369-X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/21548331.1991.11704126"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00070-09"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1005384"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/322697"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00450-4"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.2.302-317.2000"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00051.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61808-X"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61187-8"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70027-1"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.1.66"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198502143120704"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199001000-00016"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit250"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61186-6"},{"key":"e_1_3_3_19_2","first-page":"765","article-title":"Identification of group B streptococci using 16S rRNA, cfb, scpB, and atr genes in pregnant women by PCR","volume":"54","author":"Mousavi SM","year":"2016","unstructured":"Mousavi SM, Hosseini SM, Mashouf RY, Arabestani MR. 2016. 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