{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:20:56Z","timestamp":1792552856286,"version":"4.5.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Resistance of\n                    <jats:named-content content-type=\"genus-species\">Bordetella pertussis<\/jats:named-content>\n                    to erythromycin has been increasingly reported. We developed an allele-specific PCR method for rapid detection of erythromycin-resistant\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    directly from nasopharyngeal (NP) swab samples submitted for diagnostic PCR. Based on the proven association of erythromycin resistance with the A2047G mutation in the 23S rRNA of\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    , four primers, two of which were designed to be specific for either the wild-type or the mutant allele, were used in two different versions of the allele-specific PCR assay. The methods were verified with results obtained by PCR-based sequencing of 16 recent\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    isolates and 100 NP swab samples submitted for diagnostic PCR. The detection limits of the two PCR assays ranged from 10 to 100 fg per reaction for both erythromycin-susceptible and -resistant\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    . Two amplified fragments of each PCR, of 286 and 112 bp, respectively, were obtained from a mutant allele of the isolates and\/or NP swab samples containing\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    DNAs. For the wild-type allele, only a 286-bp fragment was visible when the allele-specific PCR assay 1 was performed. No amplification was found when a number of non-\n                    <jats:named-content content-type=\"genus-species\">Bordetella<\/jats:named-content>\n                    bacterial pathogens and NP swab samples that did not contain the DNAs of\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    were examined. This assay can serve as an alternative for PCR-based sequencing, especially for local laboratories in resource-poor countries.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01499-15","type":"journal-article","created":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T23:14:20Z","timestamp":1438211660000},"page":"3418-3422","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Direct Detection of Erythromycin-Resistant Bordetella pertussis in Clinical Specimens by PCR"],"prefix":"10.1128","volume":"53","author":[{"given":"Zengguo","family":"Wang","sequence":"first","affiliation":[{"name":"The Fourth Military Medical University, Xi'an, China"},{"name":"Xi'an Center for Disease Prevention and Control, Xi'an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijun","family":"Han","sequence":"additional","affiliation":[{"name":"Xi'an Center for Disease Prevention and Control, Xi'an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Xi'an Center for Disease Prevention and Control, Xi'an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanli","family":"Du","sequence":"additional","affiliation":[{"name":"Xi'an Center for Disease Prevention and Control, Xi'an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jifeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Xi'an Center for Disease Prevention and Control, Xi'an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaofeng","family":"Ma","sequence":"additional","affiliation":[{"name":"Xi'an Center for Disease Prevention and Control, Xi'an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengxin","family":"Li","sequence":"additional","affiliation":[{"name":"Xi'an Center for Disease Prevention and Control, Xi'an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiushui","family":"He","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Capital Medical University, Beijing, China"},{"name":"Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Yan","sequence":"additional","affiliation":[{"name":"The Fourth Military Medical University, Xi'an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1","article-title":"Recommended antimicrobial agents for the treatment and postexposure prophylaxis of pertussis: 2005 CDC guidelines","volume":"54","author":"Tiwari T","year":"2005","unstructured":"Tiwari T, Murphy TV, Moran J, National Immunization Program, CDC. 2005. Recommended antimicrobial agents for the treatment and postexposure prophylaxis of pertussis: 2005 CDC guidelines. MMWR Recomm Rep 54:1\u201316.","journal-title":"MMWR Recomm Rep"},{"key":"e_1_3_2_3_2","first-page":"807","article-title":"Erythromycin-resistant Bordetella pertussis\u2013Yuma County, Arizona, May\u2013October 1994","volume":"43","author":"Anonymous","year":"1994","unstructured":"Anonymous. 1994. Erythromycin-resistant Bordetella pertussis\u2013Yuma County, Arizona, May\u2013October 1994. MMWR Morb Mortal Wkly Rep 43:807\u2013810.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1167-1172.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1806.120091"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12161"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01081-13"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12671"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.326-382.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12357"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02759"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm343"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.2008\/002857-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0976-1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.09.017"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2014.04.001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.5812\/jjm.10880"},{"key":"e_1_3_2_18_2","first-page":"975","article-title":"Clinical characteristics of whooping cough in neonates and antimicrobial resistance of the pathogenic bacteria","volume":"16","author":"Luo J","year":"2014","unstructured":"Luo J, Wang HX, Yuan L, Gu S, Jiang M, Ding YJ, Guo D, Yao KH, Wang YJ. 2014. Clinical characteristics of whooping cough in neonates and antimicrobial resistance of the pathogenic bacteria. Zhongguo Dang Dai Er Ke Za Zhi 16:975\u2013978. (In Chinese.)","journal-title":"Zhongguo Dang Dai Er Ke Za Zhi"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.16.6749"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47602-0"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01499-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01499-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T03:44:30Z","timestamp":1646451870000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01499-15"}},"subtitle":[],"editor":[{"given":"M. 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