{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T02:36:45Z","timestamp":1785465405894,"version":"3.56.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Primary screening using high-risk human papillomavirus (hrHPV) detection has been suggested as a way of improving cervical cancer prevention. Women currently not attending screening (nonresponders) are more likely to participate when given the opportunity of self-sampling for hrHPV testing. The Evalyn Brush is a new cervicovaginal self-sampling device, developed specifically to meet women's demands, which is user-friendly and easy to use. The aims of this study were to investigate agreement of hrHPV detection by two PCR methods between the Evalyn Brush and physician-obtained samples and to study women's acceptance of this self-sampling device. Each of 134 women visiting the gynecology outpatient clinic collected a self-obtained sample (self-sample) and completed a questionnaire. The brush was stored dry. After self-sampling, a trained physician obtained a conventional cervical cytology specimen in ThinPrep medium. HrHPV detection was performed using the SPF\n            <jats:sub>10<\/jats:sub>\n            -DEIA-LiPA\n            <jats:sub>25<\/jats:sub>\n            and GP5+\/6+-LQ-test. The overall agreement for hrHPV detection using SPF\n            <jats:sub>10<\/jats:sub>\n            -DEIA-LiPA\n            <jats:sub>25<\/jats:sub>\n            between the self-sample and the physician-taken sample was 85.8% (kappa value, 0.715; 95% confidence interval [CI], 0.597 to 0.843;\n            <jats:italic>P<\/jats:italic>\n            = 1.000). The overall agreement for hrHPV detection using GP5+\/6+-LQ between the self-sample and the physician-taken sample was 86.6% (kappa value, 0.725; 95% CI, 0.607 to 0.843;\n            <jats:italic>P<\/jats:italic>\n            = 0.815). Ninety-eight percent of the women rated their experience as good to excellent. Moreover, 95% of women preferred self-sampling to physician sampling. Self-sampling using the dry Evalyn Brush system is as good as a physician-taken sample for hrHPV detection and is highly acceptable to women. To validate this self-sampling device for clinical use, a large screening cohort should be studied.\n          <\/jats:p>","DOI":"10.1128\/jcm.01506-12","type":"journal-article","created":{"date-parts":[[2012,9,27]],"date-time":"2012-09-27T16:07:52Z","timestamp":1348762072000},"page":"3937-3943","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Dry Storage and Transport of a Cervicovaginal Self-Sample by Use of the Evalyn Brush, Providing Reliable Human Papillomavirus Detection Combined with Comfort for Women"],"prefix":"10.1128","volume":"50","author":[{"given":"Romy","family":"van Baars","sequence":"first","affiliation":[{"name":"DDL Diagnostic Laboratory, Rijswijk, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remko P.","family":"Bosgraaf","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bram W. A.","family":"ter Harmsel","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Reinier de Graaf Groep, Voorburg, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem J. G.","family":"Melchers","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim G. V.","family":"Quint","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory, Rijswijk, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruud L. 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