{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T11:40:15Z","timestamp":1784547615020,"version":"3.55.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Haemophilu<\/jats:named-content>\n            s\n            <jats:named-content content-type=\"genus-species\">haemolyticus<\/jats:named-content>\n            has been recently discovered to have the potential to cause invasive disease. It is closely related to nontypeable\n            <jats:named-content content-type=\"genus-species\">Haemophilus influenzae<\/jats:named-content>\n            (NT\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            ). NT\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            are often misidentified because none of the existing tests targeting the known phenotypes of\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            are able to specifically identify\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            . Through comparative genomic analysis of\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            and NT\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            , we identified genes unique to\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            that can be used as targets for the identification of\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            . A real-time PCR targeting\n            <jats:italic>purT<\/jats:italic>\n            (encoding phosphoribosylglycinamide formyltransferase 2 in the purine synthesis pathway) was developed and evaluated. The lower limit of detection was 40 genomes\/PCR; the sensitivity and specificity in detecting\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            were 98.9% and 97%, respectively. To improve the discrimination of\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            and NT\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            , a testing scheme combining two targets (\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            <jats:italic>purT<\/jats:italic>\n            and\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            <jats:italic>hpd<\/jats:italic>\n            , encoding protein D lipoprotein) was also evaluated and showed 96.7% sensitivity and 98.2% specificity for the identification of\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            and 92.8% sensitivity and 100% specificity for the identification of\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            , respectively. The dual-target testing scheme can be used for the diagnosis and surveillance of infection and disease caused by\n            <jats:named-content content-type=\"genus-species\">H. haemolyticus<\/jats:named-content>\n            and NT\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.01511-16","type":"journal-article","created":{"date-parts":[[2016,10,6]],"date-time":"2016-10-06T02:59:05Z","timestamp":1475722745000},"page":"3010-3017","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Comparative Genomic Analysis of Haemophilus haemolyticus and Nontypeable Haemophilus influenzae and a New Testing Scheme for Their Discrimination"],"prefix":"10.1128","volume":"54","author":[{"given":"Fang","family":"Hu","sequence":"first","affiliation":[{"name":"Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lavanya","family":"Rishishwar","sequence":"additional","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Applied Bioinformatics Laboratory, Atlanta, Georgia, USA"},{"name":"PanAmerican Bioinformatics Institute, Cali, Valle del Cauca, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambily","family":"Sivadas","sequence":"additional","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel J.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Institute of Science and Technology Austria, Klosterneuburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. King","family":"Jordan","sequence":"additional","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Applied Bioinformatics Laboratory, Atlanta, Georgia, USA"},{"name":"PanAmerican Bioinformatics Institute, Cali, Valle del Cauca, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy F.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Clinical and Translational Research Center, Department of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet R.","family":"Gilsdorf","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard W.","family":"Mayer","sequence":"additional","affiliation":[{"name":"Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06575-11"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01832-07"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-93-1-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir735"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00089-15"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/509824"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01255-10"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00566-12"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00069-10"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02191-13"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00013-09"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt189"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00664"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.11.004"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034083"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00345-15"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06702-11"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05863-11"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1062"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"e_1_3_3_22_2","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N","year":"1987","unstructured":"Saitou N, Nei M. 1987. 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