{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T18:46:27Z","timestamp":1791744387806,"version":"4.3.4"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In July 2006, an Australian tourist returning from Dubai, in the United Arab Emirates (UAE), developed acute scrub typhus. Her signs and symptoms included fever, myalgia, headache, rash, and eschar.\n                    <jats:italic>Orientia tsutsugamushi<\/jats:italic>\n                    serology demonstrated a 4-fold rise in antibody titers in paired serum collections (1:512 to 1:8,192), with the sera reacting strongest against the Gilliam strain antigen. An\n                    <jats:italic>Orientia<\/jats:italic>\n                    species was isolated by the\n                    <jats:italic>in vitro<\/jats:italic>\n                    culture of the patient's acute blood taken prior to antibiotic treatment. The gene sequencing of the 16S rRNA gene (\n                    <jats:italic>rrs<\/jats:italic>\n                    ), partial 56-kDa gene, and the full open reading frame 47-kDa gene was performed, and comparisons of this new\n                    <jats:italic>Orientia<\/jats:italic>\n                    sp. isolate to previously characterized strains demonstrated significant sequence diversity. The closest homology to the\n                    <jats:italic>rrs<\/jats:italic>\n                    sequence of the new\n                    <jats:italic>Orientia<\/jats:italic>\n                    sp. isolate was with three strains of\n                    <jats:italic>O. tsutsugamushi<\/jats:italic>\n                    (Ikeda, Kato, and Karp), with a nucleotide sequence similarity of 98.5%. The closest homology to the 47-kDa gene sequence was with\n                    <jats:italic>O. tsutsugamushi<\/jats:italic>\n                    strain Gilliam, with a nucleotide similarity of 82.3%, while the closest homology to the 56-kDa gene sequence was with\n                    <jats:italic>O. tsutsugamushi<\/jats:italic>\n                    strain TA686, with a nucleotide similarity of 53.1%. The molecular divergence and geographically unique origin lead us to believe that this organism should be considered a novel species. Therefore, we have proposed the name \u201c\n                    <jats:italic>Orientia chuto<\/jats:italic>\n                    ,\u201d and the prototype strain of this species is strain Dubai, named after the location in which the patient was infected.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01526-10","type":"journal-article","created":{"date-parts":[[2010,10,6]],"date-time":"2010-10-06T22:40:39Z","timestamp":1286404839000},"page":"4404-4409","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":280,"title":["Isolation of a Novel\n                    <i>Orientia<\/i>\n                    Species (\n                    <i>O. chuto<\/i>\n                    sp. nov.) from a Patient Infected in Dubai"],"prefix":"10.1128","volume":"48","author":[{"given":"Leonard","family":"Izzard","sequence":"first","affiliation":[{"name":"The Australian Rickettsial Reference Laboratory\/Barwon Biomedical Research, The Geelong Hospital, Bellarine Street, Geelong, Victoria, Australia 3220"},{"name":"Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, South Street Murdoch, Western Australia 6156, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Fuller","sequence":"additional","affiliation":[{"name":"The Alfred Hospital, Melbourne, Victoria, Australia 3000"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D.","family":"Blacksell","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420\/6 Rajvithi Road, Bangkok 10400, Thailand"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel H.","family":"Paris","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420\/6 Rajvithi Road, Bangkok 10400, Thailand"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen L.","family":"Richards","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420\/6 Rajvithi Road, Bangkok 10400, Thailand"},{"name":"Viral and Rickettsial Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, Maryland 20910-7500"},{"name":"Department of Preventive Medicine &amp; Biometrics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4799"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuntipa","family":"Aukkanit","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420\/6 Rajvithi Road, Bangkok 10400, Thailand"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chelsea","family":"Nguyen","sequence":"additional","affiliation":[{"name":"The Australian Rickettsial Reference Laboratory\/Barwon Biomedical Research, The Geelong Hospital, Bellarine Street, Geelong, Victoria, Australia 3220"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Jiang","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, Maryland 20910-7500"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stan","family":"Fenwick","sequence":"additional","affiliation":[{"name":"Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, South Street Murdoch, Western Australia 6156, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P. J.","family":"Day","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420\/6 Rajvithi Road, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Graves","sequence":"additional","affiliation":[{"name":"The Australian Rickettsial Reference Laboratory\/Barwon Biomedical Research, The Geelong Hospital, Bellarine Street, Geelong, Victoria, Australia 3220"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Stenos","sequence":"additional","affiliation":[{"name":"The Australian Rickettsial Reference Laboratory\/Barwon Biomedical Research, The Geelong Hospital, Bellarine Street, Geelong, Victoria, Australia 3220"},{"name":"Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, South Street Murdoch, Western Australia 6156, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"420","volume":"20","year":"1996","unstructured":"Currie, B., D. Lo, P. Marks, G. Lum, P. Whelan, and V. Krause. 1996. Fatal scrub typhus from Litchfield Park, Northern Territory. Clin. Infect. Dis. 20 : 420-421.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb08791.x"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5456-5465.2003"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1991.tb120994.x"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.786"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1996.54.647"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.55.749"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/596576"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1995.tb07372.x"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-40-4-323"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.73.1083"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-3-589"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/11.3.237"},{"key":"e_1_3_1_16_2","unstructured":"Rickettsial diseases 2007"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200310000-00009"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01526-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01526-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T03:34:48Z","timestamp":1646451288000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01526-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":16,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1128\/JCM.01526-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01526-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12]]},"assertion":[{"value":"2010-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}