{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T01:02:26Z","timestamp":1785286946986,"version":"3.55.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A commercially available, nonamplified, nucleic acid probe-based test system (BD Affirm VPIII) was compared with nucleic acid amplification (NAA)-based assays for determining the etiology of vaginitis in a cohort of 323 symptomatic women. First, a semiquantitative, multiplexed PCR assay (BV-PCR) and the Affirm VPIII\n            <jats:italic>Gardnerella<\/jats:italic>\n            <jats:italic>vaginalis<\/jats:italic>\n            test were compared with a unified bacterial-vaginosis (BV) reference standard incorporating both Nugent Gram stain scores and Amsel clinical criteria. In the evaluable population of 305 patients, BV-PCR was 96.9% (191\/197) sensitive and 92.6% specific (100\/108) for BV, while Affirm VPIII was 90.1% sensitive (179\/197) and 67.6% specific (73\/108). Second, a multiplexed PCR assay detecting\n            <jats:named-content content-type=\"genus-species\">Candida albicans<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Candida glabrata<\/jats:named-content>\n            (CAN-PCR) was compared with the Affirm VPIII\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            test using a reference standard for vulvovaginal candidiasis (VVC) of yeast culture plus exclusion of alternate vaginitis etiologies. In the population evaluated (\n            <jats:italic>n<\/jats:italic>\n            = 102), CAN-PCR was 97.7% sensitive (42\/43) and 93.2% specific (55\/59) and Affirm VP III was 58.1% sensitive (25\/43) and 100% specific (59\/59) for VVC. Finally, the results of a commercial NAA test (GenProbe Aptima\n            <jats:named-content content-type=\"genus-species\">Trichomonas vaginalis<\/jats:named-content>\n            assay; ATV) for\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            were compared with the Affirm VPIII\n            <jats:named-content content-type=\"genus-species\">Trichomonas vaginalis<\/jats:named-content>\n            test. In the absence of an independent reference standard for trichomonal vaginitis (TV), a positive result in either assay was deemed to represent true infection. In the evaluable cohort of 388 patients, the sensitivity of ATV was 98.1% (53\/54) versus 46.3% (25\/54) for Affirm VPIII. The diagnostic accuracy of the combined NAA-based test construct was approximately 20 to 25% higher than that of the Affirm VPIII when modeled in populations with various prevalences of infectious vaginitis.\n          <\/jats:p>","DOI":"10.1128\/jcm.01537-13","type":"journal-article","created":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T04:28:22Z","timestamp":1377750502000},"page":"3694-3699","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Comparison of Nucleic Acid Amplification Assays with BD Affirm VPIII for Diagnosis of Vaginitis in Symptomatic Women"],"prefix":"10.1128","volume":"51","author":[{"given":"Charles P.","family":"Cartwright","sequence":"first","affiliation":[{"name":"ViroMed Laboratories Inc., Minnetonka, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryndon D.","family":"Lembke","sequence":"additional","affiliation":[{"name":"ViroMed Laboratories Inc., Minnetonka, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalpana","family":"Ramachandran","sequence":"additional","affiliation":[{"name":"ViroMed Laboratories Inc., Minnetonka, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara A.","family":"Body","sequence":"additional","affiliation":[{"name":"Center for Esoteric Testing, Laboratory Corporation of America Holdings, Burlington, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda B.","family":"Nye","sequence":"additional","affiliation":[{"name":"Center for Esoteric Testing, Laboratory Corporation of America Holdings, Burlington, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Rivers","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane R.","family":"Schwebke","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(12)90722-X"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-010-0096-1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2010.07.001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-7125(16)30496-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02367-10"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.1.148-152.1994"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/1064744042000210375"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/IPC.0b013e318210fdc0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP7TBN5VZUGLZU"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00506-12"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/588661"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.10.005"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2006.01312.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00748-12"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(83)91112-9"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.2.297-301.1991"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00076-09"},{"key":"e_1_3_2_19_2","first-page":"6","volume-title":"BD Affirm\u2122 VPIII Microbial Identification Test package insert","author":"Becton Dickinson and Company","year":"2010","unstructured":"Becton Dickinson and Company. 2010. BD Affirm\u2122 VPIII Microbial Identification Test package insert, p 6. Becton Dickinson and Company, Sparks, MD."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01272-07"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.3057-3059.2002"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-008-0681-9"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2006.01284.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02485-07"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2011.06.017"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01537-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01537-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:29:46Z","timestamp":1646479786000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01537-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":24,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1128\/JCM.01537-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01537-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"2013-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}