{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:49:31Z","timestamp":1792734571215,"version":"4.5.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid, accurate method for identifying bacteria and fungi recovered on agar culture media. We report herein a method for the direct identification of bacteria in positive blood culture broths by MALDI-TOF mass spectrometry. A total of 212 positive cultures were examined, representing 32 genera and 60 species or groups. The identification of bacterial isolates by MALDI-TOF mass spectrometry was compared with biochemical testing, and discrepancies were resolved by gene sequencing. No identification (spectral score of &lt;1.7) was obtained for 42 (19.8%) of the isolates, due most commonly to insufficient numbers of bacteria in the blood culture broth. Of the bacteria with a spectral score of \u22651.7, 162 (95.3%) of 170 isolates were correctly identified. All 8 isolates of\n            <jats:italic>Streptococcus mitis<\/jats:italic>\n            were misidentified as being\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            isolates. This method provides a rapid, accurate, definitive identification of bacteria within 1 h of detection in positive blood cultures with the caveat that the identification of\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            would have to be confirmed by an alternative test.\n          <\/jats:p>","DOI":"10.1128\/jcm.01541-09","type":"journal-article","created":{"date-parts":[[2009,12,3]],"date-time":"2009-12-03T03:10:27Z","timestamp":1259809827000},"page":"444-447","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":335,"title":["Rapid Identification of Bacteria in Positive Blood Culture Broths by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry"],"prefix":"10.1128","volume":"48","author":[{"given":"Lindsay G.","family":"Stevenson","sequence":"first","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven K.","family":"Drake","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick R.","family":"Murray","sequence":"additional","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02405-06"},{"key":"e_1_3_2_3_2","unstructured":"CLSI document M35-A2. 2008"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2007.00649.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00556-07"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-004-1112-x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(199808)33:8<750::AID-JMS680>3.0.CO;2-1"},{"key":"e_1_3_2_8_2","first-page":"30","volume":"22","year":"2009","unstructured":"Hensley, D. M., R. Tapia, and Y. Encina. 2009. An evaluation of the Advandx Staphylococcus aureus\/CNS PNA FISH assay. Clin. Lab. Sci. 22 : 30-33.","journal-title":"Clin. Lab. 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