{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:56:43Z","timestamp":1777654603158,"version":"3.51.4"},"reference-count":9,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Viable\n            <jats:named-content content-type=\"genus-species\">Bordetella pertussis<\/jats:named-content>\n            isolates are essential for surveillance purposes. We performed culture of 223 PCR-positive nasopharyngeal samples.\n            <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n            was recovered from 45 (20.2%) of the samples. Growth was associated with a high bacterial load, as determined by PCR. Culture from PCR-positive samples is a feasible approach to recover\n            <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n            isolates, and culture can be limited to samples with a high bacterial load.\n          <\/jats:p>","DOI":"10.1128\/jcm.01553-12","type":"journal-article","created":{"date-parts":[[2012,10,4]],"date-time":"2012-10-04T05:36:11Z","timestamp":1349328971000},"page":"4114-4115","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Recovery of Bordetella pertussis from PCR-Positive Nasopharyngeal Samples Is Dependent on Bacterial Load"],"prefix":"10.1128","volume":"50","author":[{"given":"Didrik F.","family":"Vestrheim","sequence":"first","affiliation":[{"name":"Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Steinbakk","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha L.","family":"Bj\u00f8rnstad","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir","family":"Moghaddam","sequence":"additional","affiliation":[{"name":"F\u00fcrst Medical Laboratory, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nils","family":"Reinton","sequence":"additional","affiliation":[{"name":"F\u00fcrst Medical Laboratory, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette L.","family":"Dahl","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Vestfold Hospital Trust, T\u00f8nsberg, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nils","family":"Grude","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Vestfold Hospital Trust, T\u00f8nsberg, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"Sandven","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1139\/m92-200"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-50-5-436"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.11.065"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01838-08"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00612-12"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2005.05.002"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.10.007"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.6.3.303-309.1977"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2011.622715"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01553-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01553-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:08:23Z","timestamp":1646478503000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01553-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":9,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1128\/JCM.01553-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01553-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]},"assertion":[{"value":"2012-06-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-09-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}