{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:02:07Z","timestamp":1790143327686,"version":"4.0.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Neurocysticercosis (NC), caused by the larval stage of\n            <jats:italic>Taenia solium<\/jats:italic>\n            , is one of the most common parasitic diseases of the central nervous system. The diagnosis of NC is mostly based on costly brain neuroimaging (computed tomography and\/or nuclear magnetic resonance), which is rarely accessible in most affected areas. The most sensitive and specific tools for NC diagnosis are imagery techniques. The identification of specific antibodies and antigens is currently used only to support NC diagnosis due to their limited specificity and sensitivity. This study was performed to compare immunodiagnostic assays (antibody detection by enzyme-linked immunosorbent assay [ELISA] and enzyme-linked immunoelectrotransfer blotting [EITB] and HP10 antigen detection by ELISA) with the detection of parasite DNA by PCR amplification of a repetitive element of the parasite genome in the cerebrospinal fluid (CSF) of 121 radiologically and clinically characterized NC patients. Patients were divided into six groups according to the stage of the parasites and their localization. The CSF cellularity of each patient was also recorded. When all patients were considered, PCR exhibited the highest sensitivity (95.9%) and variable specificity (80% or 100%) depending on the controls used. The sensitivities of antibody detection by ELISA and EITB were not significantly different, and ELISA identified HP10 antigen mostly when vesicular cysticerci were located in the subarachnoideal basal cisterns. These results can help in the selection of different individual assays or combinations of assays to be used in NC diagnosis according to different requirements.\n          <\/jats:p>","DOI":"10.1128\/jcm.01554-10","type":"journal-article","created":{"date-parts":[[2010,11,11]],"date-time":"2010-11-11T03:52:43Z","timestamp":1289447563000},"page":"195-200","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Human Neurocysticercosis: Comparison of Different Diagnostic Tests Using Cerebrospinal Fluid"],"prefix":"10.1128","volume":"49","author":[{"given":"Lorraine","family":"Michelet","sequence":"first","affiliation":[{"name":"EA3174 Neuroe\u0301pide\u0301miologie Tropicale et Compare\u0301e, Universite\u0301 de Limoges, Faculte\u0301 de Me\u0301decine, 2 rue du Dr Marcland, 87025 Limoges"},{"name":"Laboratoire de Parasitologie-Mycologie, AP-HP, Groupe Hospitalier Pitie\u0301-Salpe\u0302trie\u0300re, 47 boulevard de l'Ho\u0302pital, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agne\u0300s","family":"Fleury","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Neurologia y Neurocirugia, SSA, Insurgentes Sur 3877, Colonia La Fama, Mexico, DF 14269"},{"name":"Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edda","family":"Sciutto","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Kendjo","sequence":"additional","affiliation":[{"name":"UMR S 945 Immunite\u0301 et Infection, CHU Pitie\u0301-Salpe\u0302trie\u0300re\u2014Universite\u0301 Pierre et Marie Curie, 91 boulevard de l'Ho\u0302pital"},{"name":"Department of Parasitology, Institute of Tropical Medicine, University of Tu\u0308bingen, Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gladis","family":"Fragoso","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Paris","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, AP-HP, Groupe Hospitalier Pitie\u0301-Salpe\u0302trie\u0300re, 47 boulevard de l'Ho\u0302pital, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Bouteille","sequence":"additional","affiliation":[{"name":"EA3174 Neuroe\u0301pide\u0301miologie Tropicale et Compare\u0301e, Universite\u0301 de Limoges, Faculte\u0301 de Me\u0301decine, 2 rue du Dr Marcland, 87025 Limoges"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Almeida C. 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