{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:02:49Z","timestamp":1787166169002,"version":"build-2736575974"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001704","name":"European Society of Clinical Microbiology and Infectious Diseases","doi-asserted-by":"crossref","award":["\u201cIndividual Research Grant\u201d, 2024"],"award-info":[{"award-number":["\u201cIndividual Research Grant\u201d, 2024"]}],"id":[{"id":"10.13039\/501100001704","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,3,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Genomic surveillance of pathogens is essential to trace infections and analyze resistance markers. Core genome multilocus sequence typing (cgMLST) facilitates genomic surveillance by simplified analysis and standardization. However, its application is limited by the poor cost-efficiency of short-read (SR) sequencing. Oxford Nanopore long-read sequencing (ONT-LR), which allows fast on-site analysis with comparatively low costs, could provide an alternative. Despite ONT-LR raw read accuracy improvement, evidence for methylation-based errors accumulates. PCR-based library preparation, suggested as a solution, presumably poses difficulties for GC-rich bacteria. We challenged ONT-LR-based cgMLST using the highly GC-rich pathogen\n                      <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                      to develop a clinically applicable workflow. Our\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      cgMLST scheme was applied to ONT-LR data, and the results were validated against SR data. Native, rapid, and PCR-based library preparation was performed and combined with different basecalling models (SUP@bacterial-methylation, SUP@v4.2, SUP@v4.3, and SUP@v5.0) and polishing strategies (medaka_consensus, medaka_variant, r103_min_high_g360). To ensure reliability across genotypes, we included 14 sequence types and 27 genotypes. The recommended ONT-LR workflow at study initiation (SUP@v4.2, medaka_consensus) showed nearly 200 allele differences compared with the reference for specific strains. PCR-based library preparation resulted in missing targets and typing errors of up to 21 alleles. Native barcoding with SUP@v5.0 basecalling and r103_min_high_g360 polishing outperformed the PCR-based approach in all parameters reducing the error rate to a maximum of two allele differences. The optimized ONT-LR-based cgMLST workflow for\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      integrates high resolution and ease of implementation with enhanced cost-efficiency for rapid diagnostics. The developed protocol might serve as a guideline for other GC-rich pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      This study highlights a significant advancement in genomic surveillance of bacterial pathogens, specifically addressing the challenges posed by the GC-rich species\n                      <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                      . Core genome multilocus sequence typing (cgMLST) is widely used for bacterial typing as it combines high resolution with simple implementation and standardization. To improve cost efficiency and thus accessibility, we changed the sequencing approach from Illumina short-read (SR) to Oxford Nanopore long-read sequencing (ONT-LR). ONT-LR-based cgMLST showed a very high error rate compared with SR-based cgMLST, most likely due to methylation-associated errors. PCR-based library preparation, which is proposed to correct these errors, did not achieve the required accuracy. In contrast, native barcoding with advanced basecalling and polishing strategies massively reduces allelic differences. This optimized ONT-LR cgMLST workflow provides a transformative solution for cost-efficient, high-resolution typing of\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      . Furthermore, this study can serve as a guide for similarly challenging bacteria.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.01569-24","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T09:00:41Z","timestamp":1738832441000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Improving Nanopore sequencing-based core genome MLST for global infection control: a strategy for GC-rich pathogens like\n                    <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1128","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8363-0434","authenticated-orcid":false,"given":"Sarah","family":"Weigl","sequence":"first","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Dabernig-Heinz","sequence":"additional","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Granitz","sequence":"additional","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Lipp","sequence":"additional","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ostermann","sequence":"additional","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dag","family":"Harmsen","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Operative Dentistry, University Hospital M\u00fcnster","place":["M\u00fcnster, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanh Trung","family":"Trinh","sequence":"additional","affiliation":[{"name":"VNU Institute of Microbiology and Biotechnology, Vietnam National University","place":["Hanoi, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0510-7336","authenticated-orcid":true,"given":"Ivo","family":"Steinmetz","sequence":"additional","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5704-3955","authenticated-orcid":true,"given":"Gabriel E.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5668-7786","authenticated-orcid":true,"given":"Sabine","family":"Lichtenegger","sequence":"additional","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0214-5"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-023-00649-y"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr1813907"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2017.88"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30939-7"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01472-w"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00093-21"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01987-17"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00086-20"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01725-23"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001126"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01631-22"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00628-24"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.066"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.711437"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","unstructured":"Tyson JR James P Stoddart D Sparks N Wickenhagen A Hall G Choi JH Lapointe H Kamelian K Smith AD Prystajecky N Goodfellow I Wilson SJ Harrigan R Snutch TP Loman NJ Quick J. 2017 Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore. Genomics. doi:10.1101\/2020.09.04.283077","DOI":"10.1101\/2020.09.04.283077"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00945-23"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01539-7"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-023-09343-z"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0257521"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-023-05605-4"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","unstructured":"Lohde M Wagner GE Dabernig-Heinz J Viehweger A Braun SD Monecke S Diezel C Stein C Marquet M Ehricht R Pletz MW Brandt C. 2024. Accurate bacterial outbreak tracing with Oxford Nanopore sequencing and reduction of methylation-induced errors. Genome Res 34:2039\u20132047. doi:10.1101\/gr.278848.123","DOI":"10.1101\/gr.278848.123"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed3020039"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijregi.2024.100377"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13122548"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0072-8"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-018-1540-z"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2522"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1009802"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.01569-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.01569-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T18:51:00Z","timestamp":1743619860000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.01569-24"}},"subtitle":[],"editor":[{"given":"Daniel J.","family":"Diekema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,12]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3,12]]}},"alternative-id":["10.1128\/jcm.01569-24"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01569-24","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,12]]},"assertion":[{"value":"2024-10-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01569-24"}}