{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:24:54Z","timestamp":1791156294463,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid detection and differentiation of\n            <jats:italic>Taenia<\/jats:italic>\n            species are required for the control and prevention of taeniasis and cysticercosis in areas where these diseases are endemic. Because of the lower sensitivity and specificity of the conventional diagnosis based on microscopical examination, molecular tools are more reliable for differential diagnosis of these diseases. In this study, we developed and evaluated a loop-mediated isothermal amplification (LAMP) assay for differential diagnosis of infections with\n            <jats:italic>Taenia<\/jats:italic>\n            species with cathepsin L-like cysteine peptidase (clp) and cytochrome\n            <jats:italic>c<\/jats:italic>\n            oxidase subunit 1 (cox1) genes. LAMP with primer sets to the cox1 gene could differentiate between three species, and LAMP with primer sets to the clp gene could differentiate\n            <jats:italic>Taenia solium<\/jats:italic>\n            from\n            <jats:italic>Taenia saginata<\/jats:italic>\n            \/\n            <jats:italic>Taenia asiatica<\/jats:italic>\n            . Restriction enzyme digestion of the LAMP products from primer set Tsag-clp allowed the differentiation of\n            <jats:italic>Taenia saginata<\/jats:italic>\n            from\n            <jats:italic>Taenia asiatica<\/jats:italic>\n            . We demonstrated the high specificity of LAMP by testing known parasite DNA samples extracted from proglottids (\n            <jats:italic>n<\/jats:italic>\n            = 100) and cysticerci (\n            <jats:italic>n<\/jats:italic>\n            = 68). LAMP could detect one copy of the target gene or five eggs of\n            <jats:italic>T. asiatica<\/jats:italic>\n            and\n            <jats:italic>T. saginata<\/jats:italic>\n            per gram of feces, showing sensitivity similar to that of PCR methods. Furthermore, LAMP could detect parasite DNA in all taeniid egg-positive fecal samples (\n            <jats:italic>n<\/jats:italic>\n            = 6). Due to the rapid, simple, specific, and sensitive detection of\n            <jats:italic>Taenia<\/jats:italic>\n            species, the LAMP assays are valuable tools which might be easily applicable for the control and prevention of taeniasis and cysticercosis in countries where these diseases are endemic.\n          <\/jats:p>","DOI":"10.1128\/jcm.01573-08","type":"journal-article","created":{"date-parts":[[2008,11,13]],"date-time":"2008-11-13T01:58:25Z","timestamp":1226541505000},"page":"168-174","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":99,"title":["Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of\n            <i>Taenia<\/i>\n            Species"],"prefix":"10.1128","volume":"47","author":[{"given":"Agathe","family":"Nkouawa","sequence":"first","affiliation":[{"name":"Department of Parasitology"},{"name":"Medical Research Center, Institute of Medical Research and Medicinal Plants Studies (IMPM), Ministry of Scientific Research and Innovation, Yaounde\u0301, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhito","family":"Sako","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Nakao","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Nakaya","sequence":"additional","affiliation":[{"name":"Animal Laboratory for Medical Research, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510 Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.12.4463-4470.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000060686"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1309.061148"},{"key":"e_1_3_2_5_2","first-page":"1203","volume":"29","year":"1996","unstructured":"Bern, C., H. 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OIE, Paris, France.","journal-title":"WHO\/FAO\/OIE guidelines for the surveillance, prevention and control of taeniasis\/cysticercosis."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2005.10.005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2005.03.008"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652006000100010"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.057901"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.04.016"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2005.00670.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2007.06.018"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2005.06.031"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.548-553.2004"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01573-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01573-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:41:13Z","timestamp":1646462473000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01573-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1128\/JCM.01573-08"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01573-08","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1]]},"assertion":[{"value":"2008-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-11-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}