{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:17:45Z","timestamp":1792624665418,"version":"4.5.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            From June to September 2012, 500 urine samples were recovered from patients with urinary tract infections (UTI) due to Gram-negative bacilli (\u226510\n            <jats:sup>4<\/jats:sup>\n            leukocytes\/ml and \u226510\n            <jats:sup>5<\/jats:sup>\n            Gram-negative isolates\/ml) who visited the University hospital Bic\u00eatre (France). They were challenged with extended-spectrum-\u03b2-lactamase (ESBL)-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            (ESBL-E) using the rapid diagnostic ESBL NDP test. Results of the ESBL NDP test were compared to the results of the double-disc susceptibility test (DDST) performed on solid-agar plates and molecular identification of the \u03b2-lactamase genes. Among the 450 nonduplicate urine samples, 11.3% were positive for ESBL-E by using the DDST, the ESBL determinants being mostly of the CTX-M type (CTX-M-15) according to molecular testing. Results of the ESBL NDP test were obtained within 15 min. The sensitivity and specificity of the ESBL NDP test were 98% and 99.8%, respectively, whereas the positive and negative predictive values of this test were 98% and 99.8%, respectively. A perfect correlation between cefotaxime resistance and positivity of the ESBL NDP test was observed. Therefore, the ESBL NDP test offers a powerful tool for a rapid identification of ESBL-E and associated resistance to expanded-spectrum cephalosporins. It may be useful in particular for guiding first-line antibiotic therapy.\n          <\/jats:p>","DOI":"10.1128\/jcm.01578-14","type":"journal-article","created":{"date-parts":[[2014,8,7]],"date-time":"2014-08-07T06:05:27Z","timestamp":1407391527000},"page":"3701-3706","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Rapid Detection of Extended-Spectrum-\u03b2-Lactamase-Producing Enterobacteriaceae from Urine Samples by Use of the ESBL NDP Test"],"prefix":"10.1128","volume":"52","author":[{"given":"Laurent","family":"Dortet","sequence":"first","affiliation":[{"name":"INSERM U914, Le Kremlin-Bic\u00eatre, France"},{"name":"Centre National Associ\u00e9-Centre de R\u00e9f\u00e9rence des R\u00e9sistances aux Antibiotiqus, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"INSERM U914, Le Kremlin-Bic\u00eatre, France"},{"name":"Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"INSERM U914, Le Kremlin-Bic\u00eatre, France"},{"name":"Centre National Associ\u00e9-Centre de R\u00e9f\u00e9rence des R\u00e9sistances aux Antibiotiqus, Le Kremlin-Bic\u00eatre, France"},{"name":"Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"},{"name":"H\u00f4pital Fribourgeois-h\u00f4pital Cantonal, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1","article-title":"Ambulatory medical care utilization estimates for 2007","volume":"2011","author":"Schappert SM","year":"2011","unstructured":"SchappertSMRechtsteinerEA. 2011. Ambulatory medical care utilization estimates for 2007. Vital. Health Stat. 13 2011:1\u201338.","journal-title":"Vital. Health Stat. 13"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1104429"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/319757"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/500663"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00859-12"},{"key":"e_1_3_2_7_2","first-page":"1","volume-title":"Clinical microbiology procedure handbook","author":"Pezzlo M","year":"2004","unstructured":"PezzloMYorkMK. 2004. Urine cultures, p 1\u201331. In IsenbergHD (ed), Clinical microbiology procedure handbook. 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