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In this report, we provide evidence of parvovirus infection and replication in the brains of five 6-week-old Cretan hound puppies suffering from a puppy shaker syndrome and leukoencephalopathy. Although these puppies belonged to two different litters, they were closely related, tracing back two generations to the same sire. Histologically, a mild to moderate lymphohistiocytic meningitis, with focal lymphohistiocytic leukoencephalitis in two animals, and a mild to moderate vacuolation with myelin loss, mainly in the white matter of the cerebellum was detected. Vacuolation was also found in the corpus callosum, fimbria hippocampi, mesencephalon, capsula interna, basal ganglia, and hypothalamus. By immunohistology and<jats:italic>in situ<\/jats:italic>hybridization, either parvoviral antigen, DNA, mRNA, or replicative intermediate DNA were detected in the cerebellum, hippocampus, periventricular areas, corpus callosum, cerebral cortex, medulla oblongata, and spinal cord. Parvovirus antigen, DNA, and mRNA were present in cells of the outer granular layer of the cerebellum and in periventricular cells, most likely representing spongioblasts, glial cells, neurons, endothelial cells, occasional macrophages, and ependymal cells. Sequencing revealed canine parvovirus type 2 stretches. Thus, an association of parvovirus infection with the leukoencephalopathy seems likely, possibly facilitated by a genetic predisposition due to the mode of inbreeding in this particular dog breed.<\/jats:p>","DOI":"10.1128\/jcm.01582-09","type":"journal-article","created":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T02:37:21Z","timestamp":1277951841000},"page":"3169-3175","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Leukoencephalopathy Associated with Parvovirus Infection in Cretan Hound Puppies"],"prefix":"10.1128","volume":"48","author":[{"given":"D.","family":"Schaudien","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Polizopoulou","sequence":"additional","affiliation":[{"name":"Clinic of Companion Animal Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Koutinas","sequence":"additional","affiliation":[{"name":"Clinic of Companion Animal Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Schwab","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Porombka","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Baumga\u0308rtner","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Herden","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1354\/vp.36-1-77"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(01)00770-5"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05905-0"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.388"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.54.3.316-329.1990"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00688706"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/589863"},{"key":"e_1_3_1_9_2","unstructured":"Clinical neurology in small animals: localization diagnosis and treatment 2003"},{"key":"e_1_3_1_10_2","first-page":"93","volume":"23","year":"2000","unstructured":"Buonavoglia, D., A. 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