{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:57:09Z","timestamp":1792457829270,"version":"4.5.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            spp. are among the most common causes of opportunistic invasive fungal infections in tertiary care hospitals. Little is known about the prevalence and\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility of\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            species in Latin America, because there are few medical centers able to perform accurate identification at the species level. The purpose of this study was to analyze the distribution of cryptic and rare\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            species among clinical samples from 133 patients with suspected aspergillosis admitted in 12 medical centers in Brazil and to analyze the\n            <jats:italic>in vitro<\/jats:italic>\n            activity of different antifungal drugs. The identification of\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            species was performed based on a polyphasic approach, as well as sequencing analysis of the internal transcribed spacer (ITS) region, calmodulin, and \u03b2-tubulin genes and phylogenetic analysis when necessary. The\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility tests with voriconazole, posaconazole, and itraconazole were performed according to the CLSI M38-A2 document (2008). We demonstrated a high prevalence of cryptic species causing human infection. Only three isolates, representing the species\n            <jats:named-content content-type=\"genus-species\">Aspergillus thermomutatus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">A. ochraceus<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">A. calidoustus<\/jats:named-content>\n            , showed less\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility to at least one of the triazoles tested. Accurate identifications of\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            at the species level and with\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility tests are important because some species may present unique resistance patterns against specific antifungal drugs.\n          <\/jats:p>","DOI":"10.1128\/jcm.01582-14","type":"journal-article","created":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T02:54:29Z","timestamp":1406775269000},"page":"3633-3640","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":90,"title":["Cryptic and Rare Aspergillus Species in Brazil: Prevalence in Clinical Samples and\n            <i>In Vitro<\/i>\n            Susceptibility to Triazoles"],"prefix":"10.1128","volume":"52","author":[{"given":"C. E.","family":"Negri","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Universidade Federal de S\u00e3o Paulo, Escola Paulista de Medicina, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. S.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Universidade Federal de S\u00e3o Paulo, Escola Paulista de Medicina, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Xafranski","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Universidade Federal de S\u00e3o Paulo, Escola Paulista de Medicina, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. D.","family":"Bergamasco","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Universidade Federal de S\u00e3o Paulo, Escola Paulista de Medicina, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. R.","family":"Aquino","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. T. O.","family":"Castro","sequence":"additional","affiliation":[{"name":"Hospital de C\u00e2ncer de Barretos, Barretos, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L.","family":"Colombo","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Universidade Federal de S\u00e3o Paulo, Escola Paulista de Medicina, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/525258"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2006.06.001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612811319200005"},{"key":"e_1_3_2_5_2","volume-title":"Atlas of clinical fungi","author":"De Hoog GS","year":"2011","unstructured":"De HoogGSGuarroJFiguerasMJ. 2011. Atlas of clinical fungi. CBS-KNAW Fungal Biodiversity Centre, Utrecht, the Netherlands. http:\/\/www.clinicalfungi.org."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.1.4.435"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06822.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3114\/sim.2007.59.09"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2012.08.006"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3852\/mycologia.100.2.205"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.499916"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01902-12"},{"key":"e_1_3_2_13_2","volume-title":"Introduction to food and airborne fungi","author":"Samson RA","year":"2004","unstructured":"SamsonRAHoekstraESFrisvadJC (ed). 2004. Introduction to food and airborne fungi, 7th ed. Centraalbureau voor Schimmelcultures, Utrecht, the Netherlands.","edition":"7"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1323-1330.1995"},{"key":"e_1_3_2_15_2","volume-title":"Phylogenetic analysis using parsimony (and other methods), version 4.0b 10 (Alvitec)","author":"Swofford DL","year":"2002","unstructured":"SwoffordDL. 2002. PAUP. Phylogenetic analysis using parsimony (and other methods), version 4.0b 10 (Alvitec). Sinauer Associates, Sunderland, MA."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.8.754"},{"key":"e_1_3_2_17_2","first-page":"289","volume-title":"Experimental and molecular approaches to plant biosystematics","author":"Baum DA","year":"1995","unstructured":"BaumDAShawKL. 1995. Genealogical perspectives on the species problem, p 289\u2013303. In HochPCStephensonAG (ed), Experimental and molecular approaches to plant biosystematics. Missouri Botanical Garden, St Louis, MO."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.2000.1228"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.5598\/imafungus.2012.03.01.07"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1554\/03-073"},{"key":"e_1_3_2_21_2","volume-title":"Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard, 2nd ed. CLSI document M38-A2. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"2"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02432-10"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/2047-783X-16-4-153"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1086\/518852"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00692-10"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05763.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100121528"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.512-517.2005"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01070-09"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00383-13"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00535-08"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.3114\/sim.2007.59.05"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00585-09"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00425-07"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01582-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01582-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:41:49Z","timestamp":1646480509000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01582-14"}},"subtitle":[],"editor":[{"given":"D. J.","family":"Diekema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,10]]},"references-count":33,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1128\/JCM.01582-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01582-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10]]},"assertion":[{"value":"2014-06-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}