{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:18:38Z","timestamp":1792192718670,"version":"4.4.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Streptococcus suis<\/jats:named-content>\n                    is an emerging zoonotic pathogen causing severe infections in pigs and humans. Thirty-three serotypes of\n                    <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n                    have been identified using serum agglutination. The capsular polysaccharides synthesis (\n                    <jats:italic>cps<\/jats:italic>\n                    ) locus is usually conserved among different strains of the same serotype. The\n                    <jats:italic>cps<\/jats:italic>\n                    loci of 15 serotypes have been sequenced, while the loci of the other serotypes remain unknown. In the present study, two to six serotype-specific genes of each of eight serotypes, i.e., serotypes 3, 4, 5, 8, 10, 19, 23, and 25, were identified using cross-hybridization with 93 nucleic acid probes specific to genes in the\n                    <jats:italic>cps<\/jats:italic>\n                    locus, and serotype-specific PCR assays for rapid and sensitive detection of the eight serotypes were then developed. The PCR typing results of the 148 serologically typeable isolates were completely consistent with agglutination results. Furthermore, some autoagglutinating, acapsular, and multiagglutinating strains which could not be differentiated by traditional serum agglutination assays were positive in the PCR assays. Use of the PCR assays with clinical tonsillar specimens showed that the assays are sensitive and able to identify samples with autoagglutinating isolates. To our knowledge, this is the first study to identify the serotype-specific genes of the eight\n                    <jats:named-content content-type=\"genus-species\">Streptococcus suis<\/jats:named-content>\n                    serotypes and develop rapid and sensitive PCR assays for the eight serotypes which can be identified only by serum agglutination.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01584-12","type":"journal-article","created":{"date-parts":[[2012,8,9]],"date-time":"2012-08-09T02:04:01Z","timestamp":1344477841000},"page":"3329-3334","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Development of Rapid Serotype-Specific PCR Assays for Eight Serotypes of Streptococcus suis"],"prefix":"10.1128","volume":"50","author":[{"given":"Kaicheng","family":"Wang","sequence":"first","affiliation":[{"name":"Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"},{"name":"China Animal Health and Epidemiology Center, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqiang","family":"Sun","sequence":"additional","affiliation":[{"name":"China Animal Health and Epidemiology Center, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengping","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00147-3"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020031"},{"key":"e_1_3_3_4_2","first-page":"417","article-title":"Genetics of capsular polysaccharide production in bacteria","volume":"43","author":"Boulnois GJ","year":"1989","unstructured":"BoulnoisGJ. 1989. 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