{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:32:42Z","timestamp":1790400762470,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Both for epidemiologic studies and for diagnostic testing, there is a need for effective, economical, and readily available selective media for the culture of\n            <jats:italic>Clostridium difficile<\/jats:italic>\n            . We have developed a reduced-cost substitute for cycloserine-cefoxitin-fructose agar (CCFA), which is an effective but expensive selective medium for\n            <jats:italic>C. difficile<\/jats:italic>\n            . The modified medium, called\n            <jats:italic>C. difficile<\/jats:italic>\n            brucella agar (CDBA), includes an enriched brucella base as a substitute for proteose peptone no. 2, and the concentration of sodium taurocholate has been reduced from 0.1% to 0.05%. To compare the sensitivities and selectivities of CDBA and CCFA, cultures for\n            <jats:italic>C. difficile<\/jats:italic>\n            were performed using stool samples from patients with C.\n            <jats:italic>difficile<\/jats:italic>\n            -associated disease. CDBA was as sensitive as CCFA for the recovery of\n            <jats:italic>C. difficile<\/jats:italic>\n            , with a similar frequency of breakthrough growth of stool microflora (25% versus 31%, respectively). A liquid formulation of the modified medium, termed\n            <jats:italic>C. difficile<\/jats:italic>\n            brucella broth (CDBB), stimulated rapid germination and outgrowth of\n            <jats:italic>C. difficile<\/jats:italic>\n            spores, at a rate comparable to that in cycloserine-cefoxitin-fructose broth. Our results suggest that CDBA and CDBB are sensitive, selective, and reduced-cost media for the recovery of\n            <jats:italic>C. difficile<\/jats:italic>\n            from stool samples.\n          <\/jats:p>","DOI":"10.1128\/jcm.01591-08","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T02:10:52Z","timestamp":1228961452000},"page":"397-400","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Effective and Reduced-Cost Modified Selective Medium for Isolation of\n            <i>Clostridium difficile<\/i>"],"prefix":"10.1128","volume":"47","author":[{"given":"Michelle M.","family":"Nerandzic","sequence":"first","affiliation":[{"name":"Research Service"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Curtis J.","family":"Donskey","sequence":"additional","affiliation":[{"name":"Research Service"},{"name":"Geriatric Research, Education and Clinical Center, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.45.9.812"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/1.3.530"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/labmed\/22.5.335"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.21.4.636-637.1985"},{"key":"e_1_3_2_6_2","first-page":"1201","volume":"54","year":"2005","unstructured":"Centers for Disease Control and Prevention. 2005. 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L., and P. Setlow. 1990. Sporulation, germination and outgrowth, p. 391-450. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons Ltd., Chichester, England.","journal-title":"Molecular biological methods for Bacillus."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.1031189"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/521854"},{"key":"e_1_3_2_21_2","first-page":"161","year":"1993","unstructured":"Summanen, P., E. J. Baron, D. M. Citron, C. A. Strong, H. M. Wexler, and S. M. Finegold. 1993. Preparation of media and reagents, p. 161-181. In S. Hoffman (ed.), Wadsworth anaerobic bacteriology manual, 5th ed. Star Publishing Company, Belmont, CA.","journal-title":"Wadsworth anaerobic bacteriology manual"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.1999.0253"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.18.4.1017-1019.1983"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.15.3.443-446.1982"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01591-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01591-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:44:16Z","timestamp":1646462656000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01591-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1128\/JCM.01591-08"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01591-08","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2]]},"assertion":[{"value":"2008-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-12-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}