{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T13:05:22Z","timestamp":1771851922828,"version":"3.50.1"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Chagas' disease caused by\n            <jats:italic>Trypanosoma cruzi<\/jats:italic>\n            is endemic in Latin America.\n            <jats:italic>T. cruzi<\/jats:italic>\n            presents heterogeneous populations and comprises two main genetic lineages, named\n            <jats:italic>T. cruzi<\/jats:italic>\n            I and\n            <jats:italic>T. cruzi<\/jats:italic>\n            II. Diagnosis in the chronic phase is based on conventional serological tests, including indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA), and diagnosis in the acute phase based on parasitological methods, including hemoculture. The objective of this study was to evaluate the diagnostic procedures of Chagas' disease in adult patients in the chronic phase by using a PCR assay and conventional serological tests, including TESA-blot as the gold standard. Samples were obtained from 240 clinical chronic chagasic patients. The sensitivities, compared to that of TESA-blot, were 70% for PCR using the kinetoplast region, 75% for PCR using the nuclear repetitive region, 99% for IIF, and 95% for ELISA. According to the serological tests results, we recommend that researchers assess the reliability and sensitivity of the commercial kit Chagatest ELISA recombinant, version 3.0 (Chagatest Rec v3.0; Wiener Lab, Rosario, Argentina), due to the lack of sensitivity. Based on our analysis, we concluded that PCR cannot be validated as a conventional diagnostic technique for Chagas' disease. These data have been corroborated by low levels of concordance with serology test results. It is recommended that PCR be used only for alternative diagnostic support. Using the nuclear repetitive region of\n            <jats:italic>T. cruzi<\/jats:italic>\n            , PCR could also be applicable for monitoring patients receiving etiologic treatment.\n          <\/jats:p>","DOI":"10.1128\/jcm.01601-09","type":"journal-article","created":{"date-parts":[[2009,10,22]],"date-time":"2009-10-22T01:45:18Z","timestamp":1256175918000},"page":"3945-3951","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Evaluation of Adult Chronic Chagas' Heart Disease Diagnosis by Molecular and Serological Methods"],"prefix":"10.1128","volume":"47","author":[{"given":"Juan David","family":"Rami\u0301rez","sequence":"first","affiliation":[{"name":"Centro de Investigaciones en Microbiologi\u0301a y Parasitologi\u0301a Tropical, CIMPAT, Facultad de Ciencias, Departamento de Ciencias Biolo\u0301gicas, Universidad de los Andes, Carrera 1 No. 18A 10, Bogota\u0301, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe","family":"Guhl","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones en Microbiologi\u0301a y Parasitologi\u0301a Tropical, CIMPAT, Facultad de Ciencias, Departamento de Ciencias Biolo\u0301gicas, Universidad de los Andes, Carrera 1 No. 18A 10, Bogota\u0301, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eufrosina Setsu","family":"Umezawa","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical de Sa\u0303o Paulo, Faculdade de Medicina da Universidade de Sa\u0303o Paulo, CEP 05403-000, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos A.","family":"Morillo","sequence":"additional","affiliation":[{"name":"McMaster University, Population Health Research Institute, Hamilton, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Rosas","sequence":"additional","affiliation":[{"name":"Electrofisiologi\u0301a, Cli\u0301nica Abood Shaio, Bogota\u0301, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose A.","family":"Marin-Neto","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Medical School of Ribeira\u0303o Preto, Universidade de Sa\u0303o Paulo, AV Bandeirantes, 3900 Ribeira\u0303o Preto, Sa\u0303o Paulo, Brazil 14048-900"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Laboratorio de Micologi\u0301a y Fitopatologi\u0301a Universidad de los Andes, LAMFU\u2014Facultad de Ciencias, Departamento de Ciencias Biolo\u0301gicas, Universidad de los Andes, Carrera 1 No. 18A 10, Bogota\u0301, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2003.10.017"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90116-N"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761993000100030"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1995.1139"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00127-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-002-0679-3"},{"key":"e_1_3_2_8_2","first-page":"775","volume":"10","year":"2003","unstructured":"Chiurillo, M. 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