{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T21:33:35Z","timestamp":1779917615032,"version":"3.53.1"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,5,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Malaria and babesiosis are significant parasitic infections, requiring timely diagnosis to avoid severe complications. This study retrospectively compared hematology thin smears (HS) with parasitology thick and thin blood smears (PS), the gold standard for diagnosis, to evaluate HS\u2019s performance in detecting\n              <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n              and\n              <jats:italic toggle=\"yes\">Babesia<\/jats:italic>\n              infections. Of 529 cases with paired HS and PS testing, HS demonstrated 93.3% sensitivity and 99.8% specificity, with 97.7% positive and 99.4% negative predictive values. When only considering new diagnoses, HS and PS were 100% concordant. No significant difference was found in percent parasitemia between HS and PS, highlighting HS as a reliable diagnostic tool in settings where PS or other diagnostic modalities may not be readily available.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                This study demonstrates that hematology thin smears\u2014often available in laboratories that may not have other means of diagnosing blood parasite infections such as parasitology thick and thin smears, rapid diagnostics tests, or polymerase chain reaction\u2014are an accurate and reliable way to diagnose\n                <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                and\n                <jats:italic toggle=\"yes\">Babesia<\/jats:italic>\n                infections in a low prevalence setting.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/jcm.01601-24","type":"journal-article","created":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T15:17:12Z","timestamp":1742915832000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Hematology thin smears perform equally to parasitology thick and thin blood smears for the diagnosis of\n            <i>Plasmodium<\/i>\n            and\n            <i>Babesia<\/i>\n            infections in a low prevalence setting"],"prefix":"10.1128","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5069-0945","authenticated-orcid":true,"given":"Janmesh","family":"Patel","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Wisconsin School of Medicine and Public Health","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Schuett","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, UW Health, University Hospital","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1647-0205","authenticated-orcid":true,"given":"Derrick J.","family":"Chen","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, UW Health, University Hospital","place":["Madison, USA"]},{"name":"Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-020-00224-z"},{"key":"e_1_3_2_3_2","unstructured":"About babesiosis. 2024. Available from: https:\/\/www.cdc.gov\/babesiosis\/about\/index.html?CDC_AAref_Val=https:\/\/www.cdc.gov\/ parasites\/babesiosis\/data-statistics.html"},{"key":"e_1_3_2_4_2","volume-title":"Statpearls","author":"Buck E","year":"2024","unstructured":"Buck E, Finnigan NA. 2024. Malaria. In Statpearls"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2015.02.008"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7211a1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0406"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1006659"},{"key":"e_1_3_2_9_2","unstructured":"Alternative and novel drug based prevention approaches. n.d. Available from: https:\/\/www.cdc.gov\/malaria\/php\/public-health-strategy\/alternative-drug-prevention.html?CDC_AAref_Val=https:\/\/www.cdc.gov\/malaria\/diagnosis_treatment\/clinicians1.html"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","unstructured":"Varma SL Chavan SS. 2019. Detection of malaria parasite based on thick and thin blood smear images using local binary pattern 810:967\u2013975. doi:10.1007\/978-981-13-1513-8_98","DOI":"10.1007\/978-981-13-1513-8_98"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4103\/0377-4929.48917"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/605436"},{"key":"e_1_3_2_13_2","first-page":"1746","article-title":"Laboratory diagnosis of malaria by conventional peripheral blood smear examination with Quantitative Buffy Coat (QBC) and Rapid Diagnostic Tests (RDT) - a comparative study","volume":"4","author":"Bhat S","year":"2012","unstructured":"Bhat S, Sastry A, Nagaraj E, Sharadadevi M, Sastry A. 2012. Laboratory diagnosis of malaria by conventional peripheral blood smear examination with Quantitative Buffy Coat (QBC) and Rapid Diagnostic Tests (RDT) - a comparative study. Int J Collab Res Intern Med Public Health 4:1746\u20131755.","journal-title":"Int J Collab Res Intern Med Public Health"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2022.04.060"},{"key":"e_1_3_2_15_2","first-page":"54","article-title":"Comparative study of thick smear, thin smear, QBC and antigen card test in diagnosis of malaria","volume":"17","author":"Ebrahim J","year":"2013","unstructured":"Ebrahim J, Mohammed AG, Al-Amri M, Mamdouh AG, Ramprasad N, Khan F. 2013. Comparative study of thick smear, thin smear, QBC and antigen card test in diagnosis of malaria. Int J Pure Appl Sci Technol 17:54\u201359.","journal-title":"Int J Pure Appl Sci Technol"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/jtm.12116"},{"key":"e_1_3_2_17_2","first-page":"434","article-title":"Comparative evaluation of thin smear, thick smear and antigen detection test in the diagnosis of malaria","volume":"4","author":"Dharaiya CM","year":"2021","unstructured":"Dharaiya CM. 2021. Comparative evaluation of thin smear, thick smear and antigen detection test in the diagnosis of malaria. Indian J Pathol Oncol 4:434\u2013436.","journal-title":"Indian J Pathol Oncol"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-7-22"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0293084"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01013-12"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2481-4"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12152"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00293-13"},{"key":"e_1_3_2_24_2","unstructured":"BinaxNOW Malaria. n.d. 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