{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T23:54:48Z","timestamp":1769817288178,"version":"3.49.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In this study, a novel colloidal gold-based immunochromatographic strip (ICS) containing anti-\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            capsular polysaccharide polyclonal antibodies was developed to specifically detect\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            serotypes K1 and K2. Capsular polysaccharide K1 and K2 antigens were first used to produce polyclonal anti-K1 and anti-K2 antibodies. Reference strains with different serotypes, nontypeable\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            strains, and other bacterial species were then used to assess the sensitivity and specificity of these test strips. The detection limit was found to be 10\n            <jats:sup>5<\/jats:sup>\n            CFU, and the ICSs were stable for 6 months when stored at room temperature. No false-positive or false-negative results were observed, and equivalent results were obtained compared to those of more conventional test methods, such as PCR or serum agglutination. In conclusion, the ICS developed here requires no technical expertise and allows for the specific, rapid, and simultaneous detection of\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            serotypes K1 and K2.\n          <\/jats:p>","DOI":"10.1128\/jcm.01608-16","type":"journal-article","created":{"date-parts":[[2016,10,6]],"date-time":"2016-10-06T02:59:05Z","timestamp":1475722745000},"page":"3018-3021","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Klebsiella pneumoniae Serotypes K1 and K2"],"prefix":"10.1128","volume":"54","author":[{"given":"L. Kristopher","family":"Siu","sequence":"first","affiliation":[{"name":"KeMyth Biotechnology Company, Incubation Center of Yuanpei University of Medical Technology, Hsinchu, Taiwan"},{"name":"Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan"},{"name":"Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan"},{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Kuo","family":"Tsai","sequence":"additional","affiliation":[{"name":"KeMyth Biotechnology Company, Incubation Center of Yuanpei University of Medical Technology, Hsinchu, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung-Chung","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Te-Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Phone","family":"Fung","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng-Yee","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, 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