{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T12:20:33Z","timestamp":1790943633896,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:00:00Z","timestamp":1207008000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The phylogenetically related\n            <jats:italic>Mycoplasma capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capricolum<\/jats:italic>\n            and\n            <jats:italic>M. mycoides<\/jats:italic>\n            subsp.\n            <jats:italic>mycoides<\/jats:italic>\n            biotype Large Colony are two small-ruminant pathogens involved in contagious agalactia. Their respective contributions to clinical outbreaks are not well documented, because they are difficult to differentiate with the current diagnostic techniques. In order to identify DNA sequences specific to one taxon or the other, a suppression-subtractive hybridization approach was developed. DNA fragments resulting from the reciprocal subtraction of the type strains were used as probes on a panel of\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capricolum<\/jats:italic>\n            and\n            <jats:italic>M. mycoides<\/jats:italic>\n            subsp.\n            <jats:italic>mycoides<\/jats:italic>\n            biotype Large Colony strains to assess their intrataxon specificity. Due to a high intrataxon polymorphism and important cross-reactions between taxa, a single DNA fragment was shown to be specific for\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capricolum<\/jats:italic>\n            and to be present in all\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capricolum<\/jats:italic>\n            field isolates tested in this study. A PCR assay targeting the corresponding gene (\n            <jats:italic>simp<\/jats:italic>\n            A51) was designed that resulted in a 560-bp amplification only in\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capricolum<\/jats:italic>\n            and in\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capripneumoniae<\/jats:italic>\n            (the etiological agent of contagious caprine pleuropneumonia).\n            <jats:italic>simp<\/jats:italic>\n            A51 was further improved to generate a multiplex PCR (\n            <jats:italic>mult<\/jats:italic>\n            A51) that allows the differentiation of\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capripneumoniae<\/jats:italic>\n            from\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capricolum<\/jats:italic>\n            . Both the\n            <jats:italic>simp<\/jats:italic>\n            A51 and\n            <jats:italic>mult<\/jats:italic>\n            A51 assays accurately identify\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capricolum<\/jats:italic>\n            among other mycoplasmas, including all members of the\n            <jats:italic>M. mycoides<\/jats:italic>\n            cluster.\n            <jats:italic>simp<\/jats:italic>\n            A51 and\n            <jats:italic>mult<\/jats:italic>\n            A51 PCRs are proposed as sensitive and robust tools for the specific identification of\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capricolum<\/jats:italic>\n            and\n            <jats:italic>M. capricolum<\/jats:italic>\n            subsp.\n            <jats:italic>capripneumoniae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.01617-07","type":"journal-article","created":{"date-parts":[[2008,1,31]],"date-time":"2008-01-31T02:04:48Z","timestamp":1201745088000},"page":"1307-1316","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Suppression-Subtractive Hybridization as a Strategy To Identify Taxon-Specific Sequences within the\n            <i>Mycoplasma mycoides<\/i>\n            Cluster: Design and Validation of an\n            <i>M. capricolum<\/i>\n            subsp.\n            <i>capricolum<\/i>\n            -Specific PCR Assay"],"prefix":"10.1128","volume":"46","author":[{"given":"Laure","family":"Maigre","sequence":"first","affiliation":[{"name":"AFSSA-Lyon, 31 avenue Tony Garnier, 69364 Lyon, Cedex 07, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Citti","sequence":"additional","affiliation":[{"name":"UMR1225 INRA, ENVT Ecole Nationale Ve\u0301te\u0301rinaire, 23 Chemin des Capelles BP 87614, 31076 Toulouse, Cedex 3, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Marenda","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327ois","family":"Poumarat","sequence":"additional","affiliation":[{"name":"AFSSA-Lyon, 31 avenue Tony Garnier, 69364 Lyon, Cedex 07, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Tardy","sequence":"additional","affiliation":[{"name":"AFSSA-Lyon, 31 avenue Tony Garnier, 69364 Lyon, Cedex 07, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.16.3.1062"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.136.4.98"},{"key":"e_1_3_2_4_2","first-page":"2523","volume":"128","year":"1982","unstructured":"Christiansen, C., and H. 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