{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:58:27Z","timestamp":1782979107459,"version":"3.54.5"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n            PCR (\n            <jats:italic>Tp<\/jats:italic>\n            -PCR) testing now is recommended as a valid tool for the diagnosis of primary or secondary syphilis. The objectives were to systematically review and determine the optimal specific target gene to be used for\n            <jats:italic>Tp<\/jats:italic>\n            -PCR. Comparisons of the performance of the two main targets are\n            <jats:italic>tpp47<\/jats:italic>\n            and\n            <jats:italic>polA<\/jats:italic>\n            genes were done using meta-analysis. Three electronic bibliographic databases, representing abstract books from five conferences specialized in infectious diseases from January 1990 to March 2015, were searched. Search keywords included (\u201csyphilis\u201d OR \u201c\n            <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n            \u201d OR \u201cneurosyphilis\u201d) AND (\u201cPCR\u201d OR \u201cPCR\u201d OR \u201cmolecular amplification\u201d). We included diagnostic studies assessing the performance of\n            <jats:italic>Tp<\/jats:italic>\n            -PCR targeting\n            <jats:italic>tpp47<\/jats:italic>\n            (\n            <jats:italic>tpp<\/jats:italic>\n            47-\n            <jats:italic>Tp<\/jats:italic>\n            -PCR) or the\n            <jats:italic>polA<\/jats:italic>\n            gene (\n            <jats:italic>polA-Tp<\/jats:italic>\n            -PCR) in ulcers from early syphilis. All studies were assessed against quality criteria using the QUADAS-2 tool. Of 37 studies identified, 62.2% were judged at low risk of bias or applicability. Most used the U.S. Centers for Disease Control and Prevention (CDC) case definitions for primary or secondary (early) syphilis (89.2%;\n            <jats:italic>n<\/jats:italic>\n            = 33); 15 (40.5%) used darkfield microscopy (DFM). We did not find differences in sensitivity and specificity between the two\n            <jats:italic>Tp<\/jats:italic>\n            -PCR methods in the subgroup of studies using adequate reference tests. Among studies using DFM as the reference test, sensitivities were 79.8% (95% confidence intervals [CI], 72.7 to 85.4%) and 71.4% (46.0 to 88.0%) for\n            <jats:italic>tpp47-Tp<\/jats:italic>\n            -PCR and\n            <jats:italic>polA-Tp<\/jats:italic>\n            -PCR (\n            <jats:italic>P<\/jats:italic>\n            = 0.217), respectively; respective specificities were 95.3% (93.5 to 96.6%) and 93.7% (91.8 to 95.2%) (\n            <jats:italic>P<\/jats:italic>\n            = 0.304). Our findings suggest that the two\n            <jats:italic>Tp<\/jats:italic>\n            -PCR methods have similar accuracy and could be used interchangeably.\n          <\/jats:p>","DOI":"10.1128\/jcm.01619-15","type":"journal-article","created":{"date-parts":[[2015,8,27]],"date-time":"2015-08-27T02:09:46Z","timestamp":1440641386000},"page":"3522-3529","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Comparison of Diagnostic Accuracy of PCR Targeting the 47-Kilodalton Protein Membrane Gene of Treponema pallidum and PCR Targeting the DNA Polymerase I Gene: Systematic Review and Meta-analysis"],"prefix":"10.1128","volume":"53","author":[{"given":"Ang\u00e8le","family":"Gayet-Ageron","sequence":"first","affiliation":[{"name":"Division of Clinical Epidemiology, Department of Health and Community Medicine, University of Geneva and University Hospitals of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Combescure","sequence":"additional","affiliation":[{"name":"Division of Clinical Epidemiology, Department of Health and Community Medicine, University of Geneva and University Hospitals of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Lautenschlager","sequence":"additional","affiliation":[{"name":"Triemlispital, Dermatologisches Ambulatorium Triemli, Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Ninet","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, Department of Genetics and Laboratory Medicine, University Hospitals of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas V.","family":"Perneger","sequence":"additional","affiliation":[{"name":"Division of Clinical Epidemiology, Department of Health and Community Medicine, University of Geneva and University Hospitals of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Update to public health reporting and national notification for syphilis","author":"Council of State and Territorial Epidemiologists (CSTE)","year":"2014","unstructured":"Council of State and Territorial Epidemiologists (CSTE). 2014. 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