{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:07:36Z","timestamp":1759104456508},"reference-count":18,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated the analytical, work flow, and clinical performance of the Versant CT\/GC DNA 1.0 assay (Versant CT\/GC assay, where \u201cCT\u201d represents\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            and \u201cGC\u201d represents\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            ). The assay simultaneously detects\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            in swab and first-catch urine (FCU) specimens. The limit of detection (LoD) was determined to be 342 copies\/ml for\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and 137 copies\/ml for GC. The Versant CT\/GC assay detected 15\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            serovars and 46 GC strains. The Versant CT\/GC assay demonstrated no cross-reactivity with 136 potentially cross-reacting organisms. Clinical concordance of the Versant CT\/GC assay to the Aptima Combo 2 (AC2) assay from Gen-Probe was demonstrated using 1,129 patient specimens, including 589 urine and 540 swab specimens. Discrepant specimens were subjected to DNA sequencing to identify the presence of amplified targets and to identify false-positive and false-negative results. Overall percent agreement was greater than 98%. Positive and negative percent agreements for detection of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            were 94.4% and 99.1%, respectively, in urine specimens and 95.8% and 99.8%, respectively, in swab specimens. Positive percent agreement for the detection of\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            was 100% in both urine and swab specimens, and negative percent agreements were 99.6% and 99% in urine and swab specimens, respectively. In conclusion, the performance of the Versant CT\/GC assay was comparable to that of the AC2 assay. The Versant CT\/GC assay can be recommended for the detection of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            in swab and urine specimens of symptomatic and asymptomatic individuals.\n          <\/jats:p>","DOI":"10.1128\/jcm.01634-10","type":"journal-article","created":{"date-parts":[[2011,2,10]],"date-time":"2011-02-10T03:11:32Z","timestamp":1297307492000},"page":"1347-1353","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["First Report of Performance of the Versant CT\/GC DNA 1.0 Assay (kPCR) for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae"],"prefix":"10.1128","volume":"49","author":[{"given":"P. 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