{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:51:42Z","timestamp":1785487902970,"version":"3.56.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100008672","name":"Wilhelm Sander-Stiftung","doi-asserted-by":"publisher","award":["2015-083.1"],"award-info":[{"award-number":["2015-083.1"]}],"id":[{"id":"10.13039\/100008672","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008672","name":"Wilhelm Sander-Stiftung","doi-asserted-by":"publisher","award":["2015-083.1"],"award-info":[{"award-number":["2015-083.1"]}],"id":[{"id":"10.13039\/100008672","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In patients with hematological malignancies, bronchoalveolar lavage fluid (BALF) specimens are commonly used for the diagnosis of mold infections. However, it is not clear whether the cell pellet (P) or the supernatant fraction (S) of the BALF specimen is optimal for molecular diagnostic testing. Thus, 99 BALF specimens were collected from 96 hematology patients with or without allogeneic hematopoietic stem cell transplant. The cell pellets and supernatants were processed alone and in combination (S\/P) for testing by two fungus-specific real-time PCR assays compliant with international recommendations. The results achieved with S\/P were revealed to be superior in comparison to those achieved with S and P alone, with the use of each single fraction showing a reduced sensitivity for the detection of\n                    <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n                    DNA (82% and 43% for S and P, respectively). In 57% of the samples, testing of the combination of S and P generated a lower quantification cycle value than testing of S or P alone. Molds would have been missed in 5 and 16 out of 28 samples if only S or P, respectively, was analyzed. No sample was positive by testing of S or P only. Similar results were obtained for the detection of Mucorales DNA in BALF specimens (reduced sensitivity of 67% and 50% for S and P, respectively). Study patients were categorized according to the current European Organization for the Research and Treatment of Cancer\/Mycoses Study Group classification for invasive fungal disease (IFD), revealing that 35 patients had proven\/probable IFD (36%), 47 patients had possible IFD (49%), and 14 patients had undetermined IFD (15%).\n                  <\/jats:p>","DOI":"10.1128\/jcm.01655-17","type":"journal-article","created":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T13:10:26Z","timestamp":1511961026000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["A Comparison of Aspergillus and Mucorales PCR Testing of Different Bronchoalveolar Lavage Fluid Fractions from Patients with Suspected Invasive Pulmonary Fungal Disease"],"prefix":"10.1128","volume":"56","author":[{"given":"Jan","family":"Springer","sequence":"first","affiliation":[{"name":"Medizinische Klinik und Poliklinik II, University Hospital W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Lewis","family":"White","sequence":"additional","affiliation":[{"name":"Public Health Wales Microbiology, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Kessel","sequence":"additional","affiliation":[{"name":"Medizinische Klinik II, University Hospital Frankfurt, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imke","family":"Wieters","sequence":"additional","affiliation":[{"name":"Medizinische Klinik II, University Hospital Frankfurt, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Teschner","sequence":"additional","affiliation":[{"name":"Department of Hematology, Medical Oncology, and Pneumology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Korczynski","sequence":"additional","affiliation":[{"name":"Department of Hematology, Medical Oncology, and Pneumology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Liebregts","sequence":"additional","affiliation":[{"name":"Department of Bone Marrow Transplantation, University Hospital Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver A.","family":"Cornely","sequence":"additional","affiliation":[{"name":"Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Department I of Internal Medicine, Clinical Trials Centre Cologne (ZKS K\u00f6ln), German Centre for Infection Research (DZIF), University of Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Medizinische Klinik m. S. H\u00e4matologie und Onkologie, Charit\u00e9 Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Elgeti","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Radiologie und Klinik f\u00fcr Nuklearmedizin, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Meintker","sequence":"additional","affiliation":[{"name":"Erlangen University Hospital, University of Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan W.","family":"Krause","sequence":"additional","affiliation":[{"name":"Erlangen University Hospital, University of Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel B.","family":"Posso","sequence":"additional","affiliation":[{"name":"Public Health Wales Microbiology, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner J.","family":"Heinz","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik II, University Hospital W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Fuhrmann","sequence":"additional","affiliation":[{"name":"Department I for Internal Medicine, University Hospital of Cologne, Cologne, Germany"},{"name":"German Centre for Infection Research partner site Bonn-Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg Janne","family":"Vehreschild","sequence":"additional","affiliation":[{"name":"Department I for Internal Medicine, University Hospital of Cologne, Cologne, Germany"},{"name":"German Centre for Infection Research partner site Bonn-Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Einsele","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik II, University Hospital W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Rickerts","sequence":"additional","affiliation":[{"name":"Robert Koch Institut Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6619-1100","authenticated-orcid":false,"given":"Juergen","family":"Loeffler","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik II, University Hospital W\u00fcrzburg, W\u00fcrzburg, 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