{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:30:08Z","timestamp":1791412208401,"version":"4.3.3"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Kingella kingae<\/jats:named-content>is an emerging osteoarticular pathogen in young children. Its isolation by traditional culture methods remains difficult, underscoring the need to implement other diagnostic methods for its detection and identification, such as nucleic acid amplification tests. Although the genome of this bacterium has not yet been sequenced, a toxin named RTX has been identified. The goal of this study was to develop sensitive, specific, and rapid molecular methods based on the<jats:italic>rtxA<\/jats:italic>toxin gene sequence to diagnose this infection. Two real-time PCR assays (SYBR green and TaqMan chemistries) targeting this gene are reported. Sensitivity and specificity were first evaluated successfully with 67 strains: 31<jats:named-content content-type=\"genus-species\">Kingella kingae<\/jats:named-content>isolates and 36 strains from other bacterial species. Then, 52 clinical specimens positive or negative by culture and\/or PCR (16S rRNA and<jats:italic>cpn60<\/jats:italic>genes) were tested with these assays. A nested PCR assay with subsequent sequencing was also developed to confirm the presence of<jats:named-content content-type=\"genus-species\">Kingella kingae<\/jats:named-content>isolates in these clinical specimens. The results obtained demonstrate that these assays are accurate for the diagnosis of<jats:named-content content-type=\"genus-species\">Kingella kingae<\/jats:named-content>infection.<\/jats:p>","DOI":"10.1128\/jcm.01657-10","type":"journal-article","created":{"date-parts":[[2011,1,20]],"date-time":"2011-01-20T03:27:24Z","timestamp":1295494044000},"page":"1245-1250","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["The<i>rtxA<\/i>Toxin Gene of Kingella kingae: a Pertinent Target for Molecular Diagnosis of Osteoarticular Infections"],"prefix":"10.1128","volume":"49","author":[{"given":"Philippe","family":"Lehours","sequence":"first","affiliation":[{"name":"CHU de Bordeaux, Hopital Pellegrin, Laboratoire de Bact\u00e9riologie, Bordeaux, France"},{"name":"Universit\u00e9 Victor Segalen Bordeaux 2, Laboratoire de Bact\u00e9riologie, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Marie","family":"Freydi\u00e8re","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Centre de Biologie Est, Laboratoire de Bact\u00e9riologie, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Richer","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux, H\u00f4pital des Enfants, Service de P\u00e9diatrie, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Burucoa","sequence":"additional","affiliation":[{"name":"CHU Poitiers, Laboratoire de Bact\u00e9riologie, Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Boisset","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Centre de Biologie Est, Laboratoire de Bact\u00e9riologie, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Lanotte","sequence":"additional","affiliation":[{"name":"CHRU de Tours, H\u00f4pital Bretonneau et H\u00f4pital Clocheville, Service de Bact\u00e9riologie-Virologie, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Fran\u00e7oise","family":"Pr\u00e8re","sequence":"additional","affiliation":[{"name":"CHU de Toulouse, Laboratoire de Bact\u00e9riologie, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s","family":"Ferroni","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Necker-Enfants Malades, Laboratoire de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Lafuente","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux, Hopital Pellegrin, Laboratoire de Bact\u00e9riologie, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Vandenesch","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Centre de Biologie Est, Laboratoire de Bact\u00e9riologie, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"M\u00e9graud","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux, Hopital Pellegrin, Laboratoire de Bact\u00e9riologie, Bordeaux, France"},{"name":"Universit\u00e9 Victor Segalen Bordeaux 2, Laboratoire de Bact\u00e9riologie, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armelle","family":"M\u00e9nard","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux, Hopital Pellegrin, Laboratoire de Bact\u00e9riologie, Bordeaux, France"},{"name":"Universit\u00e9 Victor Segalen Bordeaux 2, Laboratoire de Bact\u00e9riologie, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-693X(00)90005-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_3_4_2","unstructured":"Applied Biosystems. 2010 posting date. 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