{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:31:07Z","timestamp":1761129067895},"reference-count":17,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid and accurate identification of the pathogens involved in bloodstream infections is crucial for the prompt initiation of appropriate therapy, as this can decrease morbidity and mortality rates. A PCR-reverse blot hybridization assay for sepsis, the reverse blot hybridization assay (REBA) Sepsis-ID test, was developed; it uses pan-probes to distinguish Gram-positive and -negative bacteria and fungi. In addition, the assay was designed to identify bacteria and fungi using six genus-specific and 13 species-specific probes; it uses additional probes for antibiotic resistance genes, i.e., the\n            <jats:italic>mecA<\/jats:italic>\n            gene of methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            (MRSA) and the\n            <jats:italic>vanA<\/jats:italic>\n            and\n            <jats:italic>vanB<\/jats:italic>\n            genes of vancomycin-resistant enterococci (VRE). The REBA Sepsis-ID test successfully identified clinical isolates and blood culture samples as containing Gram-positive bacteria, Gram-negative bacteria, or fungi. The results matched those obtained with conventional microbiological methods. For the REBA Sepsis-ID test, of the 115 blood culture samples tested, 47 (40.8%) and 49 (42.6%) samples were identified to the species and genus levels, respectively, and the remaining 19 samples (16.5%), which included five Gram-positive rods, were identified as Gram-positive bacteria, Gram-negative bacteria, or fungi. The antibiotic resistances of the MRSA and VRE strains were identified using both conventional microbiological methods and the REBA Sepsis-ID test. In conclusion, the REBA Sepsis-ID test developed for this study is a fast and reliable test for the identification of Gram-positive bacteria, Gram-negative bacteria, fungi, and antibiotic resistance genes (including\n            <jats:italic>mecA<\/jats:italic>\n            for MRSA and the\n            <jats:italic>vanA<\/jats:italic>\n            and\n            <jats:italic>vanB<\/jats:italic>\n            genes for VRE) in bloodstream infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.01665-12","type":"journal-article","created":{"date-parts":[[2013,2,28]],"date-time":"2013-02-28T03:42:38Z","timestamp":1362022958000},"page":"1451-1457","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["PCR-Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis"],"prefix":"10.1128","volume":"51","author":[{"given":"Yeonim","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"},{"name":"Department of Biomedical Laboratory Science, Songho College, Hoengseong, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye-Young","family":"Wang","sequence":"additional","affiliation":[{"name":"M&amp;D, Inc., Wonju Eco Environmental Technology Center, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyusang","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soon-Deok","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"},{"name":"Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo-Young","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young","family":"Uh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Bae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyeyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.101.6.1644"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01326"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1053\/jinf.2002.1067"},{"key":"e_1_3_2_5_2","first-page":"265","article-title":"Microorganisms isolated from blood cultures and their antimicrobial susceptibility patterns at a university hospital during 1994\u20132003","volume":"27","author":"Koh EM","year":"2007","unstructured":"KohEM LeeSG KimCK KimM YongD LeeK KimJM KimDS ChongY. 2007. Microorganisms isolated from blood cultures and their antimicrobial susceptibility patterns at a university hospital during 1994\u20132003. Korean J. Lab Med. 27:265\u2013275. (In Korean.)","journal-title":"Korean J. Lab Med."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.3.444"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012657"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/430909"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.5026-5033.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00562-10"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir034"},{"key":"e_1_3_2_12_2","first-page":"135","article-title":"Microplate identification system of Enterobacteriaceae","volume":"2","author":"Uh Y","year":"1999","unstructured":"UhY SonJS HwangGY JangIH YoonKJ SeoDM. 1999. Microplate identification system of Enterobacteriaceae. Korean J. Clin. Microbiol. 2:135\u2013143. (In Korean.)","journal-title":"Korean J. Clin. Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00423-011-0870-z"},{"key":"e_1_3_2_14_2","first-page":"10","article-title":"Detection of waterborne pathogens by PCR-reverse blot hybridization","volume":"16","author":"Choi Y","year":"2010","unstructured":"ChoiY LeeG BangH KimJB LeeH. 2010. Detection of waterborne pathogens by PCR-reverse blot hybridization. J. Exp. Biomed. Sci. 16:10\u201318. (In Korean.)","journal-title":"J. Exp. Biomed. Sci."},{"key":"e_1_3_2_15_2","first-page":"86","article-title":"PCR-reverse blot hybridization assay for species identification of dermatophytes","volume":"16","author":"Kim H","year":"2011","unstructured":"KimH JinH KimS WangHY ChoiY BangH ParkJS LeeJH WonYH AhnKJ KimYK LeeH. 2011. PCR-reverse blot hybridization assay for species identification of dermatophytes. Korean J. Med. Mycol. 16:86\u201398. (In Korean.)","journal-title":"Korean J. Med. Mycol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022139"},{"key":"e_1_3_2_17_2","first-page":"101","article-title":"Comprehensive analysis of blood culture performed at nine university hospitals in Korea","volume":"31","author":"Shin JH","year":"2011","unstructured":"ShinJH SongSA KimMN LeeNY KimEC KimS KooSH RyooNH KimJS ChoJH. 2011. Comprehensive analysis of blood culture performed at nine university hospitals in Korea. Korean J. Lab Med. 31:101\u2013106.","journal-title":"Korean J. Lab Med."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2009.09.003"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01665-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01665-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:34:03Z","timestamp":1646480043000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01665-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["10.1128\/JCM.01665-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01665-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5]]},"assertion":[{"value":"2012-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-02-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}