{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:38:40Z","timestamp":1773286720746,"version":"3.50.1"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid detection and identification of\n            <jats:italic>Ehrlichia<\/jats:italic>\n            species improves clinical outcome for patients suspected of ehrlichiosis. We describe an assay that employs multilocus PCR and electrospray ionization mass spectrometry (PCR\/ESI-MS) to detect and identify\n            <jats:italic>Ehrlichia<\/jats:italic>\n            species directly from blood specimens. The results were compared to those of a colorimetric microtiter PCR enzyme immunoassay (PCR-EIA) used as a diagnostic assay. Among 213 whole-blood samples collected from patients who were clinically suspected of ehrlichiosis from 1 May to 1 August 2008 at Vanderbilt University Hospital, 40 were positive for an\n            <jats:italic>Ehrlichia<\/jats:italic>\n            species by PCR\/ESI-MS, giving a positive rate of 18.8%. In comparison to the PCR-EIA, PCR\/ESI-MS possessed a sensitivity, a specificity, and positive and negative predictive values of 95.0%, 98.8%, 95.0%, and 98.8%, respectively. The 38 specimens that were positive for\n            <jats:italic>Ehrlichia<\/jats:italic>\n            by both PCR\/ESI-MS and the PCR-EIA were further characterized to the species level, with 100% agreement between the two assays. In addition,\n            <jats:italic>Rickettsia rickettsii<\/jats:italic>\n            was detected by PCR\/ESI-MS from four specimens that were confirmed retrospectively by serology and PCR-EIA. In three specimens, the PCR\/ESI-MS assay identified\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            ,\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            , and\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            ; these were confirmed by culture and\/or clinical diagnosis as being clinically relevant. From specimen processing to result reporting, the PCR\/ESI-MS assay can be completed within 6 h, providing another laboratory tool for the diagnosis of ehrlichiosis. Moreover, this system may provide rapid detection and identification of additional pathogens directly from blood specimens.\n          <\/jats:p>","DOI":"10.1128\/jcm.01669-09","type":"journal-article","created":{"date-parts":[[2009,12,3]],"date-time":"2009-12-03T03:10:27Z","timestamp":1259809827000},"page":"472-478","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Detection and Identification of\n            <i>Ehrlichia<\/i>\n            Species in Blood by Use of PCR and Electrospray Ionization Mass Spectrometry"],"prefix":"10.1128","volume":"48","author":[{"given":"Mark W.","family":"Eshoo","sequence":"first","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California 92008"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris D.","family":"Crowder","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California 92008"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haijing","family":"Li","sequence":"additional","affiliation":[{"name":"Departments of Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather E.","family":"Matthews","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California 92008"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shufang","family":"Meng","sequence":"additional","affiliation":[{"name":"Departments of Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan E.","family":"Sefers","sequence":"additional","affiliation":[{"name":"Departments of Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rangarajan","family":"Sampath","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California 92008"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles W.","family":"Stratton","sequence":"additional","affiliation":[{"name":"Departments of Pathology"},{"name":"Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence B.","family":"Blyn","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California 92008"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Ecker","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California 92008"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Wei","family":"Tang","sequence":"additional","affiliation":[{"name":"Departments of Pathology"},{"name":"Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.12.2838-2842.1991"},{"key":"e_1_3_2_3_2","first-page":"299","volume":"42","year":"1992","unstructured":"Anderson, B. 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