{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T14:33:30Z","timestamp":1759847610661},"reference-count":10,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Diagnostic laboratories are under increasing pressure to improve and expand their services. Greater flexibility in sample processing is a critical factor that can improve the time to results while reducing reagent waste, making laboratories more efficient and cost-effective. The introduction of the Abbott\n m<\/jats:italic>\n PLUS feature, with the capacity for extended use of amplification reagents, significantly increases the flexibility of the\n m<\/jats:italic>\n 2000 platform and enables laboratories to customize their workflows based on sample arrival patterns. The flexibility in sample batch size offered by\n m<\/jats:italic>\n PLUS enables significant reductions in processing times. For hepatitis B virus tests, a reduction in sample turnaround times of up to 30% (105 min) was observed for batches of 12 samples compared with those for batches of 24 samples; for\n Chlamydia trachomatis<\/jats:named-content>\n \/\n Neisseria gonorrhoeae<\/jats:named-content>\n tests, the ability to run batches of 24 samples reduced the turnaround time by 83% (54 min) compared with that for batches of 48 samples. Excellent correlations between\n m<\/jats:italic>\n PLUS and\n m<\/jats:italic>\n 2000 standard condition results were observed for all RealTi\n m<\/jats:italic>\n e viral load assays evaluated in this study, with correlation\n r<\/jats:italic>\n values of 0.998 for all assays tested. For the qualitative RealTi\n m<\/jats:italic>\n e\n C. trachomatis<\/jats:named-content>\n \/\n N. gonorrhoeae<\/jats:named-content>\n assay, the overall agreements between the two conditions tested were >98% for\n C. trachomatis<\/jats:named-content>\n and 100% for\n N. gonorrhoeae<\/jats:named-content>\n . Comparable precision results were observed for the two conditions tested for all RealTi\n m<\/jats:italic>\n e assays. The enhanced\n m<\/jats:italic>\n PLUS capability provides clinical laboratories with increased efficiencies to meet increasingly stringent turnaround time requirements without increased costs associated with discarding partially used amplification reagents.\n <\/jats:p>","DOI":"10.1128\/jcm.01672-13","type":"journal-article","created":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T01:00:40Z","timestamp":1380762040000},"page":"4050-4054","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Impact of the New Abbott\n m<\/i>\n PLUS Feature on Clinical Laboratory Efficiencies of Abbott RealTi\n m<\/i>\n e Assays for Detection of HIV-1, Hepatitis C Virus, Hepatitis B Virus, Chlamydia trachomatis, and Neisseria gonorrhoeae"],"prefix":"10.1128","volume":"51","author":[{"given":"Danijela","family":"Lucic","sequence":"first","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Jones","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russ","family":"Wiesneth","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathy","family":"Barry","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Webb","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa","family":"Belova","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peggy","family":"Dolan","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiaolan","family":"Ho","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klara","family":"Abravaya","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gavin","family":"Cloherty","sequence":"additional","affiliation":[{"name":"Abbott Molecular, Des Plaines, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2012.03.008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.5858\/2007-131-1063-SAFTCC"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2011.625"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.149"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2008.085"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02231-08"},{"key":"e_1_3_2_8_2","volume-title":"Roche COBAS AmpliPrep\/COBAS TaqMan PCR instrument manual","author":"Roche Diagnostics Corp","year":"2007","unstructured":"Roche Diagnostics Corp. 2007. Roche COBAS AmpliPrep\/COBAS TaqMan PCR instrument manual. Roche Diagnostics Corp., Indianapolis, IN."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2012.07.020"},{"key":"e_1_3_2_10_2","volume-title":"Abbott m2000sp\/m2000rt instrument manual","author":"Abbott Molecular","year":"2012","unstructured":"Abbott Molecular. 2012. Abbott m2000sp\/m2000rt instrument manual. Abbott Molecular, Des Plaines, IL."},{"key":"e_1_3_2_11_2","volume-title":"Best practices for periodic maintenance of the COBAS AmpliPrep\/COBAS TaqMan test. Analyzer bulletin 07-135R","author":"Roche Diagnostics Corp","year":"2007","unstructured":"Roche Diagnostics Corp. 2007. Best practices for periodic maintenance of the COBAS AmpliPrep\/COBAS TaqMan test. Analyzer bulletin 07-135R. Roche Diagnostics Corp., Indianapolis, IN."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01672-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01672-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:27:19Z","timestamp":1646479639000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01672-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":10,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1128\/JCM.01672-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01672-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"2013-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}