{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:31:17Z","timestamp":1792020677869,"version":"4.4.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Cryptococcal meningitis is the most frequent cause of meningitis and a major cause of mortality in HIV-infected adults in Africa. This study evaluated the performance of the lateral flow assay (LFA) on cerebrospinal fluid (CSF) samples for the diagnosis of cryptococcal meningitis against that of existing diagnostic tests. LFA performed on 465 undiluted CSF samples had a sensitivity of 91%. When the LFA was paired with Gram staining, a sensitivity of 100% was achieved after implementation of a dilution step for samples with negative LFA results and the presence of yeasts on microscopy. Microscopy is essential for preventing the reporting of false-negative results due to the high-dose \u201chook\u201d effect.<\/jats:p>","DOI":"10.1128\/jcm.01683-14","type":"journal-article","created":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T03:38:14Z","timestamp":1411011494000},"page":"4172-4175","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Rapid Diagnosis of Cryptococcal Meningitis by Use of Lateral Flow Assay on Cerebrospinal Fluid Samples: Influence of the High-Dose \u201cHook\u201d Effect"],"prefix":"10.1128","volume":"52","author":[{"given":"Adr\u00e9","family":"Lourens","sequence":"first","affiliation":[{"name":"Division of Medical Microbiology, Stellenbosch University, National Health Laboratory Services, Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph N.","family":"Jarvis","sequence":"additional","affiliation":[{"name":"Department of Clinical Research, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom"},{"name":"Botswana-University of Pennsylvania Partnership, Gaborone, Botswana"},{"name":"University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme","family":"Meintjes","sequence":"additional","affiliation":[{"name":"Institute of Infectious Disease and Molecular Medicine and the Division of Infectious Diseases and HIV Medicine, Department of Medicine, University of Cape Town, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine M.","family":"Samuel","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology, Stellenbosch University, National Health Laboratory Services, Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","article-title":"Sensitivity and specificity of a new cryptococcal antigen lateral flow assay in serum and cerebrospinal fluid","volume":"45","author":"Vijayan T","year":"2013","unstructured":"VijayanTChillerTKlausnerJD. 2013. 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